Synergistic effects of physical and chemical guidance cues on neurite alignment and outgrowth on biodegradable polymer substrates
Synergistic effects of physical and chemical guidance cues on neurite alignment and outgrowth on biodegradable polymer substrates
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DOI：
10.1089/107632702760184646
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发表时间：
2002-06-01
影响因子：
--
通讯作者：
Mallapragada, S
中科院分区：
文献类型：
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作者：
Miller, C;Jeftinija, S;Mallapragada, S
本文证明，通过对可生物降解的聚合物基材应用物理和化学线索的组合，可以促进神经突的定向生长。使用专为无法直接微图案化的可生物降解聚合物开发的新型间接转移技术，对聚-D,L-乳酸和聚（丙交酯-共-乙交酯）薄膜进行微图案化，以在基材表面上形成凹槽。层粘连蛋白被选择性地吸附在凹槽中。将整个和分离的背根神经节接种在基底上，并测量神经突的生长和沿微纹的排列。微凹槽提供物理引导，而层粘连蛋白为神经元提供化学线索。发现凹槽深度和间距显着影响神经突排列。发现层粘连蛋白的存在促进神经突粘附和沿着凹槽生长。通过结合优化的物理和化学线索，获得了对定向神经突生长的出色空间控制，神经突对齐率高达 95%。发现物理和化学引导线索的协同作用比单独线索在促进神经突的定向生长方面更有效。
This article demonstrates that directional outgrowth of neurites is promoted by applying a combination of physical and chemical cues to biodegradable polymer substrates. Films of poly-D,L-lactic acid and poly(lactide-co-glycolide) were micropatterned to form grooves on substrate surfaces, using novel indirect transfer techniques developed specifically for biodegradable polymers that cannot be micropatterned directly. Laminin was selectively adsorbed in the grooves. Whole and dissociated dorsal root ganglia were seeded on the substrates and neurite outgrowth and alignment along the microgrooves were measured. The microgrooves provide physical guidance, whereas laminin provides chemical cues to the neurons. The groove depth and spacing were found to significantly influence neurite alignment. The presence of laminin was found to promote neurite adhesion and outgrowth along the grooves. Using a combination of optimized physical and chemical cues, excellent spatial control of directional neurite outgrowth, with up to 95% alignment of neurites, was obtained. The synergistic effect of physical and chemical guidance cues was found to be more effective than individual cues in promoting directional outgrowth of neurites.