RAD51 supports DMC1 by inhibiting the SMC5/6 complex during meiosis
RAD51 supports DMC1 by inhibiting the SMC5/6 complex during meiosis
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RAD51 通过在减数分裂过程中抑制 SMC5/6 复合物来支持 DMC1
DOI：
10.1093/plcell/koab136
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发表时间：
2021-05-16
期刊：
影响因子：
11.6
通讯作者：
Yan, Shunping
中科院分区：
文献类型：
--
作者：
Chen, Hanchen;He, Chengpeng;Yan, Shunping
减数分裂是大多数真核生物有性生殖的基本过程，并且进化上保守的重组酶RADiation sensitive 51（RAD 51）和Disrupted Meiotic cDNA 1（DMC 1）对于减数分裂和因此的生育力是必需的。RAD 51的有丝分裂功能是清楚的，但RAD 51的减数分裂功能在很大程度上仍然未知。在这里，我们表明，RAD 51功能作为一个相互作用的蛋白质，以抑制结构维持染色体5/6（SMC 5/6）复合物抑制DMC 1。我们意外地发现，SMC 5/6的损失部分抑制了rad 51敲除突变体的不育性，花粉活力，减数分裂染色体片段在DMC 1依赖的方式在拟南芥。生物化学和细胞学研究表明，DMC 1的减数分裂染色体定位的抑制SMC 5/6复合物，这是减弱RAD 51通过物理相互作用。本研究不仅证实了RAD 51在减数分裂中的功能，而且发现了SMC 5/6对DMC 1的抑制作用是减数分裂重组过程中的一种控制机制。
Meiosis is a fundamental process for sexual reproduction in most eukaryotes and the evolutionarily conserved recombinases RADiation sensitive51 (RAD51) and Disrupted Meiotic cDNA1 (DMC1) are essential for meiosis and thus fertility. The mitotic function of RAD51 is clear, but the meiotic function of RAD51 remains largely unknown. Here we show that RAD51 functions as an interacting protein to restrain the Structural Maintenance of Chromosomes5/6 (SMC5/6) complex from inhibiting DMC1. We unexpectedly found that loss of the SMC5/6 partially suppresses the rad51 knockout mutant in terms of sterility, pollen inviability, and meiotic chromosome fragmentation in a DMC1-dependent manner in Arabidopsis thaliana. Biochemical and cytological studies revealed that the DMC1 localization in meiotic chromosomes is inhibited by the SMC5/6 complex, which is attenuated by RAD51 through physical interactions. This study not only identified the long-sought-after function of RAD51 in meiosis but also discovered the inhibition of SMC5/6 on DMC1 as a control mechanism during meiotic recombination.