Oocyte Vitellogenesis Triggers the Entry of Yeast-Like Symbionts Into the Oocyte of Brown Planthopper (Hemiptera: Delphacidae)
Oocyte Vitellogenesis Triggers the Entry of Yeast-Like Symbionts Into the Oocyte of Brown Planthopper (Hemiptera: Delphacidae)
复制标题
卵母细胞卵黄发生触发类酵母共生体进入褐飞虱（半翅目：飞虱科）的卵母细胞
DOI：
10.1093/aesa/saw025
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发表时间：
2016-09-01
影响因子：
2.3
通讯作者：
Yu, Xiao-Ping
中科院分区：
文献类型：
--
作者：
Nan, Guo-Hui;Xu, Yi-Peng;Yu, Xiao-Ping
摘要 类酵母共生体（YLS）在褐飞虱世代之间跨卵传播，但人们对传播机制知之甚少。在本研究中，我们发现 YLS 直到褐飞虱卵母细胞发育到卵黄发生晚期才进入其体内。在卵母细胞卵黄发生过程中，卵母细胞周围滤泡细胞的多样化和形态变化与卵母细胞中卵黄蛋白原（Vg）和脂质的积累和极化有关，间接影响YLS进入卵母细胞。此外，后滤泡细胞（多样化的滤泡细胞群之一）形成上皮栓，这是 YLS 进入卵母细胞的唯一结构。此外，肌动蛋白在卵泡细胞内重新排列，为YLS从上皮栓移动到卵母细胞建立肌动蛋白桥，表明肌动蛋白介导YLS在卵母细胞卵黄发生过程中进入褐飞虱卵母细胞。综上所述，我们的结果表明，YLS 进入褐飞虱卵母细胞是由卵母细胞卵黄发生触发的。
Abstract Yeast-like symbionts (YLSs) are transovarially transmitted between brown planthopper generations, but the transmission mechanism is poorly understood. In the present study, we found that YLSs do not enter the brown planthopper oocyte until it develops to late vitellogenesis. During oocyte vitellogenesis, the diversification and morphological changes of the follicular cells surrounding the oocyte are associated with the accumulation and polarization of vitellogenin (Vg) and lipids in the oocyte, indirectly affecting the entry of YLSs into the oocyte. Additionally, the posterior follicular cells, one of the diversified follicular cell populations, form the epithelial plug that is the only structure for YLSs to enter the oocyte. In addition, actin is rearranged within the follicular cells to build an actin bridge for YLSs to move from the epithelial plug to the oocyte, indicating that actin mediates the entry of YLSs into the brown planthopper oocyte during oocyte vitellogenesis. Taken together, our results demonstrate that the entry of YLSs into the brown planthopper oocyte is triggered by oocyte vitellogenesis.