Molecular imaging with 68Ga-SSTR PET/CT and correlation to immunohistochemistry of somatostatin receptors in neuroendocrine tumours
Molecular imaging with 68Ga-SSTR PET/CT and correlation to immunohistochemistry of somatostatin receptors in neuroendocrine tumours
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DOI：
10.1007/s00259-011-1846-5
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发表时间：
2011-09-01
影响因子：
9.1
通讯作者：
Baum, Richard Paul
中科院分区：
文献类型：
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作者：
Kaemmerer, Daniel;Peter, Luisa;Baum, Richard Paul
目的生长抑素受体(SSTR)在胃肠胰腺神经内分泌肿瘤(GEP-Net)中高表达。本研究的目的是探讨由静态正电子发射断层扫描(PET/CT)产生的半定量参数所预测的受体密度是否与新的兔抗SSTR2A单抗(克隆UMB-1)用于SSTR2A特异性免疫组织化学以及SSTR1和3-5的多克隆抗体进行体外免疫组织化学的相关性。方法收集34例经手术病理证实的GEP-Net患者的14例手术标本，与术前Ga-68-DOTA-NOC PET/CT进行对照。结果SSTR2a和SSTR5的IRS与PET/CT的SUVmax呈高度正相关(p<；0.001；p<；0.05)，SSTR2a的IRS与SUV均数呈高度正相关(p<；0.013)。结论SSTR2 A与SSTR5的表达水平与肿瘤的分级呈显著正相关。结论SSTR2 A与SSTR5的表达水平与肿瘤的SUVmax呈高度正相关，与Ga-68 DOTA-NOC对SSTR亚型的亲和力相一致，表明生长抑素类似物在Net的诊断中具有良好的应用价值。这项研究将使用Ga-68-DOTA-NOC PET/CT的生长抑素受体成像与免疫组织化学分析的SSTR相关联，也强调了使用生长抑素类似物、后续成像以及放射性核素治疗的批准。
Purpose Somatostatin receptors (SSTR) are known for an overexpression in gastroenteropancreatic neuroendocrine tumours (GEP-NET). The aim of the present study was to find out if the receptor density predicted by the semi-quantitative parameters generated from the static positron emission tomography (PET/CT) correlated with the in vitro immunohistochemistry using a novel rabbit monoclonal anti-SSTR2A antibody (clone UMB-1) for specific SSTR2A immunohistochemistry and polyclonal antibodies for SSTR1 and 3-5.Methods Overall 14 surgical specimens generated from 34 histologically documented GEP-NET patients were correlated with the preoperative Ga-68-DOTA-NOC PET/CT. Quantitative assessment of the receptor density was done using the immunoreactive score (IRS) of Remmele and Stegner; the additional 4-point IRS classification for immunohistochemistry and standardized uptake values (SUVmax and SUVmean) were used for PET/CT.Results The IRS for SSTR2A and SSTR5 correlated highly significant with the SUVmax on the PET/CT (p < 0.001; p < 0.05) and the IRS for SSTR2A with the SUVmean (p < 0.013). The level of SSTR2A score correlated significantly with chromogranin A staining and indirectly to the tumour grading.Conclusion The highly significant correlation between SSTR2A and SSTR5 and the SUVmax on the Ga-68-DOTA-NOC PET/CT scans is concordant with the affinity profile of Ga-68-DOTA-NOC to the SSTR subtypes and demonstrates the excellent qualification of somatostatin analogues in the diagnostics of NET. This study correlating somatostatin receptor imaging using Ga-68-DOTA-NOC PET/CT with immunohistochemically analysed SSTR also underlines the approval of therapy using somatostatin analogues, follow-up imaging as well as radionuclide therapy.