Eugenol protects cells against oxidative stress via Nrf2.
Eugenol protects cells against oxidative stress via Nrf2.
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丁子香酚通过 Nrf2 保护细胞免受氧化应激
DOI：
10.3892/etm.2020.9539
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发表时间：
2021-03
影响因子：
2.7
通讯作者：
Yu W
中科院分区：
文献类型：
--
作者：
Ma L;Liu J;Lin Q;Gu Y;Yu W
丁香酚是一种天然存在的化合物，存在于多种植物中，并且先前已被证明发挥许多生物活性。然而，丁香酚对细胞抗氧化应激的潜在作用仍然知之甚少。本研究以HEK-293细胞和小鼠成纤维细胞系NIH-3 T3细胞为模型，探讨丁香酚对H2 O2损伤的影响。在所测试的三种天然化合物中，即丁香酚、甲基丁香酚和乙酰丁香酚，丁香酚被发现以剂量依赖性方式增加核因子红细胞2相关因子2（Nrf 2）的转录活性和表达水平，所述核因子红细胞2相关因子2（Nrf 2）是细胞对氧化应激反应的中枢调节剂。Nrf 2靶基因谷氨酸-半胱氨酸连接酶修饰物调节亚基和谷胱甘肽S-转移酶A1的mRNA水平也被发现在丁香酚处理后上调。进一步的研究表明，丁香酚增强了Nrf 2的稳定性和核转位。此外，发现用丁香酚处理以依赖于Nrf 2的方式降低细胞内ROS水平，同时增加细胞对H2 O2的抗性。总之，本研究的数据表明，丁香酚是一种抗氧化应激的保护剂，通过Nrf 2依赖性途径发挥其作用，使丁香酚及其衍生物成为未来开发抗氧化剂的有前途的候选物。
Eugenol is a naturally occurring compound that is present in a variety of plants and has previous been demonstrated to exert a number of bioactivities. However, the potential effects of Eugenol on cellular protection against oxidative stress remain poorly understood. In the present study, HEK-293 cells and the mouse fibroblast cell line NIH-3T3 cells were used as models to explore the effects of eugenol on H2O2-induced damage. Among the three natural compounds tested, namely eugenol, methyleugenol and acetyleugenol, eugenol was found to increase the transcriptional activity and expression level of nuclear factor erythroid 2-related factor 2 (Nrf2), a central regulator of cellular responses to oxidative stress, in a dose-dependent manner. The mRNA levels of Nrf2 target genes glutamate-cysteine ligase modifier regulatory subunit and glutathione S-transferase A1, were also found to be upregulated following eugenol treatment. Further study revealed that eugenol enhanced the stabilization and nuclear translocation of Nrf2. Additionally, treatment with eugenol was found to reduce intracellular ROS levels while increasing cellular resistance to H2O2, in a manner that was dependent on Nrf2. In conclusion, data from the present study suggest that eugenol is a protective agent against oxidative stress that exerts its effects through a Nrf2-dependent pathway, rendering eugenol and its derivatives to be promising candidates for the future development of antioxidants.