Pyramiding novel EMS-generated mutant alleles to improve fiber quality components of elite upland cotton germplasm
Pyramiding novel EMS-generated mutant alleles to improve fiber quality components of elite upland cotton germplasm
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DOI：
10.1016/j.indcrop.2022.114594
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发表时间：
2022-04
影响因子：
5.9
通讯作者：
Jinesh D. Patel;Rahul Chandnani;Sameer Khanal;Jeevan Adhikari;N. Brown;P. Chee;Don C. Jones;A. Paterson
中科院分区：
文献类型：
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作者：
Jinesh D. Patel;Rahul Chandnani;Sameer Khanal;Jeevan Adhikari;N. Brown;P. Chee;Don C. Jones;A. Paterson
棉花（Gossypium hirsutum L.）由于历史遗传瓶颈而受到阻碍的纤维品质，可能受益于化学诱导突变体的使用，这些突变体基本上不含通常与使用次级和三级棉基因库有关的连锁不利等位基因。通过7个纤维品质改良突变系的互交，建立了两个群体，一个群体（R群体）主要改良马克隆值、长度、比强度和伸长率，另一个群体（S群体）主要改良纤维长度的金字塔上级等位基因。两个群体的马克隆值（较低）、纤维长度、纤维强度、整齐度和短纤维指数的总体平均值与亲本相比有显著提高，其中R群体有39个品系和S群体有71个品系在4个或4个以上性状上表现出显著提高。这些群体中的多个品系显示出所有六个纤维性状的改善。群体S和R的纤维长度显著高于原始（非突变）亲本（ACALA 1517 -99和TAM 94 L25）、地方优良种质（GA 230）和其他商业对照（DeltaPine 393和Fiber Max 832）。群体S的平均纤维长度显著高于群体R的4.2%。令人惊讶的是，人口S也显着优于人口R在马克隆值，纤维强度，均匀度和短纤维含量，增加进一步支持假设的复杂性，棉花纤维数量性状基因座和推论，选择一个纤维品质性状也可能增加其他性状的值。来自这些突变株系的新等位基因组合显示出改善纤维品质的希望，超过了当前优良品种的水平。
Improvement of cotton (Gossypium hirsutumL.) fiber quality, hampered by historical genetic bottlenecks, may benefit from the use of chemical induced mutants that are largely free of linked unfavorable alleles often associated with the use of secondary and tertiaryGossypiumgene pools. Two population were developed by intercrossing seven mutant lines with improved fiber quality, one (population R) focused on improving four fiber attributes (micronaire, length, strength and elongation) and the other (population S) to pyramid superior alleles for fiber length. The overall average of both populations was significantly improved for micronaire (lower), fiber length, fiber strength, uniformity and short fiber index compared with parental lines, with 39 lines in population R and 71 in population S showing significant improvement for four or more traits. Multiple lines in these populations showed improvement for all six fiber traits tested. Fiber length of populations S and R was significantly higher than the original (non-mutated) parents (ACALA1517-99 and TAM94L25), local elite germplasm (GA230) and other commercial checks (DeltaPine 393 and Fiber Max 832). As expected, average fiber length of population S was significantly higher by 4.2% than population R. Surprisingly, population S was also significantly better than population R in micronaire, fiber strength, uniformity and short fiber content, adding further support to hypotheses about the complex nature of cotton fiber quantitative trait loci and the corollary that selection for one fiber quality trait may also increase values of other traits. New allele combinations from these mutant lines show promise for improving fiber qualities beyond the levels of current elite varieties.