Effects of glycosylated hemoglobin on vascular responses in vitro.
Effects of glycosylated hemoglobin on vascular responses in vitro.
复制标题
糖化血红蛋白对体外血管反应的影响。
DOI：
10.1016/s0008-6363(97)00016-3
复制
发表时间：
1997
影响因子：
10.8
通讯作者：
Dellsperger,KC
中科院分区：
文献类型：
--
作者：
Oltman,CL;Gutterman,DD;Scott,EC;Bocker,JM;Dellsperger,KC
血管对内皮依赖性血管扩张剂的反应在体内大大受损，而糖尿病动物的离体血管表现出不太一致的损伤程度。糖化蛋白质，如血红蛋白，已牵连在血管异常与diabetes.Objective：本研究的目的是检验假设，糖化血红蛋白是能够减少内皮依赖性血管舒张反应，可能解释受损的扩张观察到在vivo.Methods：为了检验这一假设，糖化血红蛋白（GH）对血管反应的影响进行了研究，在几个血管床，包括心室微血管和冠状动脉，肠系膜，股动脉和肾动脉。分离冠状小动脉，并将其固定在加压（30 cmH 2 O）器官室中的两个玻璃移液管之间。结果：在心室微血管中，10 nM nGH（non-GH）和GH均能减弱Ach的舒张作用。较低浓度（1 nM nGH或GH）不会改变Ach的扩张。在冠状动脉，股动脉，肠系膜和肾动脉段，内皮依赖性反应没有改变的存在下，10或100 nM的生长激素或GH.Conclusion：在冠状动脉微血管，冠状动脉，股动脉，肠系膜和肾动脉，生长激素是不负责与糖尿病相关的内皮功能受损。
Vascular responses to endothelium-dependent vasodilators are greatly impaired in vivo, while isolated blood vessels from animals with diabetes mellitus demonstrate less consistent degrees of impairment. Glycation of proteins, such as hemoglobin, has been implicated in the vascular abnormalities associated with diabetes.Objective:The purpose of this study was to test the hypothesis that glycosylated hemoglobin is capable of reducing endothelium-dependent vasodilator responses, possibly explaining impaired dilation observed in vivo.Methods:To test this hypothesis, the effect of glycosylated hemoglobin (GH) on vascular responses was studied in several vascular beds, including ventricular microvessels and coronary, mesenteric, femoral, and renal arteries. Coronary arterioles were isolated and mounted between two glass pipettes in a pressurized (30 cmH2O) organ chamber. Isolated artery segments were studied using a standard isometric ring technique.Results:In ventricular microvessels, 10 nM nGH (non-GH) and GH both attenuated the relaxation to Ach. A lower concentration, 1 nM nGH or GH, did not alter dilation to Ach. In coronary, femoral, mesenteric and renal artery segments, endothelium-dependent responses were not altered by the presence of 10 or 100 nM nGH or GH.Conclusion:In coronary microvessels, and coronary, femoral, mesenteric and renal arteries, GH is not responsible for the impaired endothelial function associated with diabetes mellitus.