THE CORRELATION BETWEEN PLASMINOGEN-ACTIVATOR ACTIVITY AND THYMIDINE INCORPORATION IN MOUSE BONE MARROW-DERIVED MACROPHAGES - OPPOSING ACTIONS OF COLONY-STIMULATING FACTOR, PHORBOL-MYRISTATE ACETATE, DEXAMETHASONE AND PROSTAGLANDIN-E
THE CORRELATION BETWEEN PLASMINOGEN-ACTIVATOR ACTIVITY AND THYMIDINE INCORPORATION IN MOUSE BONE MARROW-DERIVED MACROPHAGES - OPPOSING ACTIONS OF COLONY-STIMULATING FACTOR, PHORBOL-MYRISTATE ACETATE, DEXAMETHASONE AND PROSTAGLANDIN-E
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DOI：
10.1016/0014-4827(84)90578-0
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发表时间：
1984-01-01
影响因子：
3.7
通讯作者：
GORDON, S
中科院分区：
文献类型：
--
作者：
HUME, DA;GORDON, S
在小鼠骨髓源性巨噬细胞中研究了纤溶酶原激活剂活性和[3 H]胸苷掺入。在存在刺激性（集落刺激因子[CSF]、佛波醇肉豆蔻酸酯醋酸酯、PMA）和抑制性（地塞米松、前列腺素E1）信号的情况下，这2种活性密切相关。地塞米松和前列腺素E1的作用可以被任一刺激剂克服，因此净效应是巨噬细胞对集落刺激因子或PMA的敏感性的改变。骨髓源性巨噬细胞对CSF-1的敏感性也通过加入少量CSF-1无反应的腹腔巨噬细胞而降低。纤溶酶原激活剂诱导不是[3 H]胸苷掺入的充分信号，这需要额外的大分子血清组分。发现血清组分不是纤溶酶原。
Plasminogen activator activity and [3H]thymidine incorporation were studied in mouse bone marrow-derived macrophages. The 2 activities correlated closely in the presence of stimulatory (colony-stimulating factor [CSF], phorbol myristate acetate, PMA) and inhibitory (dexamethasone, prostaglandin E1) signals. The actions of dexamethasone and prostaglandin E1 could be overcome by either stimulatory agent, so that the net effect was an alteration in sensitivity of the macrophages to colony-stimulating factor, or PMA. The sensitivity of bone marrow-derived macrophages to CSF-1 was also reduced by the addition of small numbers of CSF-1 unresponsive peritoneal macrophages. Plasminogen activator induction was not a sufficient signal for [3H]thymidine incorporation which requires an additional macromolecular serum component. The serum component was found not to be plasminogen.