Comparative subcellular distribution of aldehyde dehydrogenase in rat, mouse and rabbit liver.
Comparative subcellular distribution of aldehyde dehydrogenase in rat, mouse and rabbit liver.
复制标题
大鼠、小鼠和兔肝脏中乙醛脱氢酶的比较亚细胞分布。
DOI：
10.1016/0006-2952(84)90109-6
复制
发表时间：
1984
影响因子：
5.8
通讯作者：
Evces,S
中科院分区：
文献类型：
--
作者：
Lindahl,R;Evces,S
本文报道了布法罗、Fischer 344、Long-Evans、Sprague-Dawley、Wistar和Purdue/Wistar大鼠肝醛脱氢酶（ALDH）活性的亚细胞分布。将这些亚细胞分布与小鼠和兔肝脏ALDH的分布进行比较。对于六种大鼠品系，在毫摩尔浓度的丙醛下，NAD依赖性ALDH活性主要与线粒体（51%）和微粒体（30%）相关。在毫摩尔乙醛浓度下，NAD依赖性ALDH主要是线粒体（高达80%）。少于1%的总NAD依赖性醛脱氢酶被发现在胞质溶胶中。高度近交的Purdue/ Wistar系具有显着降低乙酰丙酮酸-NAD ALDH活性以及降低总NADP依赖的ALDH活性比其他菌株。在CD-1小鼠肝脏中，在线粒体（60%）、微粒体（23%）和胞质溶胶（5%）中发现了毫摩尔KmNAD依赖性ALDH活性。在兔肝中，依赖于KmNAD的ALDH也分布于线粒体（36%）、微粒体（19%）和胞浆（28%）中。在微摩尔底物浓度下，线粒体具有大部分大鼠、小鼠和兔肝脏ALDH活性。在所有三个物种中，NADP依赖性ALDH活性主要存在于微粒体组分中（高达65%）。细胞质中几乎没有NADP依赖的ALDH。我们的结论是，有显着的物种之间的差异，大鼠，小鼠和兔肝醛脱氢酶的亚细胞分布。在这三个物种中，线粒体和微粒体具有大部分的肝醛脱氢酶活性。然而，小鼠和兔肝细胞质也作出了显着的贡献，总ALDH活性。对于六个大鼠品系检查，肝细胞质具有很少或没有ALDH活性。
The subcellular distribution of hepatic aldehyde dehydrogenase (ALDH) activity was determined in Buffalo, Fischer 344, Long-Evans, Sprague-Dawley, Wistar and Purdue/Wistar rats. These subcellular distributions were compared to the distribution of mouse and rabbit liver ALDH. For the six rat strains, at millimolar propionaldehyde concentrations, NAD-dependent ALDH activity was associated primarily with mitochondria (51%) and microsomes (30%). At millimolar acetaldehyde concentrations, NAD-dependent ALDH was primarily mitochondrial (up to 80%). Less than 1% of total NAD-dependent aldehyde dehydrogenase was found in the cytosol. The highly inbred Purdue/ Wistar line possessed significantly less acetaldehyde-NAD ALDH activity as well as less total NADP-dependent ALDH activity than the other strains. In CD-1 mouse liver, millimolar KmNAD-dependent ALDH activity was found in mitochondria (60%), microsomes (23%) and cytosol (5%). In rabbit liver, millimolarKmNAD-dependent ALDH was also distributed among mitochondria (36%), microsomes (19%) and cytosol (28%). At micromolar substrate concentrations, mitochondria possessed the majority of rat, mouse and rabbit liver ALDH activity. In all three species, NADP-dependent ALDH activity was found predominantly in the microsomal fraction (up to 65%). The cytosol possessed little NADP-dependent ALDH in any species. We conclude that there are significant species differences in the subcellular distribution of aldehyde dehydrogenase between rat, mouse and rabbit liver. In all three species, mitochondria and microsomes possessed the majority of hepatic aldehyde dehydrogenase activity. However, the cytosol of mouse and rabbit liver also made a significant contribution to total ALDH activity. For the six rat strains examined, liver cytosol possessed little or no ALDH activity.