Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism.
Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism.
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DOI：
10.1038/nature13995
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发表时间：
2015-01-08
期刊：
影响因子：
64.8
通讯作者：
Gilbert HJ
中科院分区：
文献类型：
--
作者：
Cuskin F;Lowe EC;Temple MJ;Zhu Y;Cameron E;Pudlo NA;Porter NT;Urs K;Thompson AJ;Cartmell A;Rogowski A;Hamilton BS;Chen R;Tolbert TJ;Piens K;Bracke D;Vervecken W;Hakki Z;Speciale G;Munōz-Munōz JL;Day A;Peña MJ;McLean R;Suits MD;Boraston AB;Atherly T;Ziemer CJ;Williams SJ;Davies GJ;Abbott DW;Martens EC;Gilbert HJ
酵母菌作为人类饮食的组成部分至少有7000年的历史，它具有精细的细胞壁α-甘露聚糖。酵母甘露聚糖对人类微生物群生态的影响尚不清楚。在这里，我们表明，酵母α-甘露聚糖是一个可行的食物来源，多形拟杆菌（Bt），一个占主导地位的成员的微生物群。详细的生化分析和靶向基因破坏研究支持一种模型，即表面上α-甘露聚糖的有限切割产生大寡糖，随后通过周质酶的作用解聚为甘露糖。共培养研究表明，Bt对酵母甘露聚糖的代谢呈现出一种“自私”的模式。该报告显示了一组非常成功的微生物群成员如何进化为消耗空间限制性酵母聚糖，这可能反映了真核微生物融入人类饮食的适应。
Yeasts, which have been a component of the human diet for at least 7000 years, possess an elaborate cell wall α-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast α-mannan is a viable food source for Bacteroides thetaiotaomicron (Bt), a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of α-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by Bt presents a ‘selfish’ model for the catabolism of this recalcitrant polysaccharide. This report shows how a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.