Expression pattern of zebrafish rxfp2 homologue genes during embryonic development.
Expression pattern of zebrafish rxfp2 homologue genes during embryonic development.
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DOI：
10.1002/jez.b.22637
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发表时间：
2015-11
期刊：
影响因子：
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通讯作者：
Aldo Donizetti;M. Fiengo;R. del Gaudio;G. Iazzetti;Paolo Pariante;S. Minucci;F. Aniello
中科院分区：
文献类型：
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作者：
Aldo Donizetti;M. Fiengo;R. del Gaudio;G. Iazzetti;Paolo Pariante;S. Minucci;F. Aniello
RXFP2是松弛素类胰岛素多肽的4种受体之一，特别是与INSL3多肽高亲和力结合。在胎盘哺乳动物的发育过程中，INSL3/RXFP2对睾丸的下降是必不可少的。这种配体/受体对的进化史引起了人们的极大关注，因为它在鱼类等缺乏睾丸后代的脊椎动物中的功能仍然难以捉摸。在此，我们分析了三个rxfp2同源基因在斑马鱼胚胎发育中的表达模式。对于所有三个rxfp2基因(rxfp2a、rxfp2b和rxfp2-like)，我们都显示了母源转录本的存在。在发育后期，rxfp2a只在幼虫阶段表达，而rxfp2b在所分析的所有阶段都有表达，在幼虫中表达最高。Rxfp2类基因在所分析的所有阶段都有表达，转录水平从咽部发育早期开始增加。Rxfp2类基因的空间定位分析表明，它在发育中的大脑中表达于许多细胞团。此外，在视网膜和口腔上皮细胞中还发现了其他类似rxfp2的表达细胞。这一分析为阐明rxfp2基因在脊椎动物谱系中的进化提供了新的见解，并为研究它们在脊椎动物胚胎发育中的作用奠定了基础。
RXFP2 is one of the 4 receptors for relaxin insulin-like peptides, in particular it binds with high affinity the INSL3 peptide. INSL3/RXFP2 pair is essential for testicular descent during placental mammalian development. The evolutionary history of this ligand/receptor pair has received much attention, since its function in vertebrate species lacking testicular descent, such as the fishes, remains elusive. Herein, we analyzed the expression pattern of three rxfp2 homologue genes in zebrafish embryonic development. For all the three rxfp2 genes (rxfp2a, rxfp2b, and rxfp2-like) we showed the presence of maternally derived transcripts. Later in the development, rxfp2a is only expressed at larval stage, whereas rxfp2b is expressed in all the analyzed stage with highest level in the larvae. The rxfp2-like gene is expressed in all the analyzed stage with a transcript level that increased starting at early pharyngula stage. The spatial localization analysis of rxfp2-like gene showed that it is expressed in many cell clusters in the developing brain. In addition, other rxfp2-like-expressing cells were identified in the retina and oral epithelium. This analysis provides new insights to elucidate the evolution of rxfp2 genes in vertebrate lineage and lays the foundations to study their role in vertebrate embryonic development.