DISTRIBUTION OF TRANSIENT RECEPTOR POTENTIAL VANILLOID 1 CHANNEL-EXPRESSING NERVE FIBERS IN MOUSE RECTAL AND COLONIC ENTERIC NERVOUS SYSTEM: RELATIONSHIP TO PEPTIDERGIC AND NITRERGIC NEURONS
DISTRIBUTION OF TRANSIENT RECEPTOR POTENTIAL VANILLOID 1 CHANNEL-EXPRESSING NERVE FIBERS IN MOUSE RECTAL AND COLONIC ENTERIC NERVOUS SYSTEM: RELATIONSHIP TO PEPTIDERGIC AND NITRERGIC NEURONS
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DOI：
10.1016/j.neuroscience.2010.10.024
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发表时间：
2011-01-13
期刊：
影响因子：
3.3
通讯作者：
Horie, S.
中科院分区：
文献类型：
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作者：
Matsumoto, K.;Hosoya, T.;Horie, S.
在肠道中，瞬时受体电位香草素(TRPV)1的激活导致神经肽和一氧化氮等神经递质的释放。然而，目前对肠道神经系统感觉神经末梢释放的TRPV1神经纤维和神经递质的分布尚不清楚。本文研究了TRPV1通道、感觉神经肽和一氧化氮在小鼠大肠中的免疫组织化学分布及其共存。大量的TRPV1和降钙素基因相关肽(CGRP)免疫反应阳性，主要分布在粘膜、粘膜下层和肌间神经丛。肌层有丰富的P物质(SP)、神经激肽A(NKA)和神经元型一氧化氮合酶(NNOS)免疫反应。对环状肌和纵行肌的运动功能研究发现，直肠以及远端、横结肠和近端结肠对辣椒素的收缩反应最敏感。在小鼠直肠水平切片上进行了双标记研究。在肌间神经丛中可见TRPV1/蛋白基因产物(PGP)9.5双标轴突，但PGP9.5和神经元核蛋白免疫阳性胞体不表达TRPV1免疫反应。在粘膜、粘膜下层、深肌丛、环肌、肌间丛和纵肌层，TRPV1神经纤维均含有CGRP、SP和nNOS。SP和NKA几乎完全共存于各层的轴突和胞体。C-Kit双标结果显示TRPV1神经纤维定位于Cajal间质细胞(ICC)附近。这些结果表明，表达TRPV1的神经及其神经递质调节大肠的各种功能。(C)2011年IBRO。爱思唯尔有限公司出版。保留所有权利。
In the gut, transient receptor potential vanilloid (TRPV) 1 activation leads to release of neurotransmitters such as neuropeptides and nitric oxide. However, the distribution of TRPV1 nerve fibers and neurotransmitters released form sensory nerve endings in the enteric nervous system are currently not well understood. The present study investigated the immunohistochemical distribution of TRPV1 channels, sensory neuropeptides, and nitric oxide and their co-localization in mouse large intestine. Numerous TRPV1 and calcitonin gene-related peptide (CGRP) immunoreactivities were detected, mainly in the mucosa, submucosal layer, and myenteric plexus. Abundant substance P (SP), neurokinin A (NKA), and neuronal nitric oxide synthase (nNOS)immunoreactivity were revealed in muscle layers. Motor function studies of circular and longitudinal muscles found that contractile responses to capsaicin in the rectum were most sensitive among the rectum, and distal, transverse, and proximal colon. Double labeling studies were carried out in horizontal sections of mouse rectum. TRPV1/protein gene product (PGP)9.5 double labeled axons were observed, but PGP9.5 and neuronal nuclear protein immunopositive cell bodies did not express TRPV1 immunoreactivity in the myenteric plexus. In the mucosa, submucosal layer, deep muscular plexus, circular muscle, myenteric plexus and longitudinal muscle layer, TRPV1 nerve fibers were found to contain CGRP, SP and nNOS. SP and NKA were almost entirely co-localized at the axons and cell bodies in all layers. Double labeling with c-Kit revealed that TRPV1 nerve fibers localized adjacent to the interstitial cells of Cajal (ICC). These results suggest that the TRPV1-expressing nerve and its neurotransmitters regulate various functions of the large intestine. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.