Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi
Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi
复制标题
DOI：
10.1038/sj.emboj.7600135
复制
发表时间：
2004-03-24
期刊：
影响因子：
11.4
通讯作者：
Tagaya, M
中科院分区：
文献类型：
--
作者：
Hirose, H;Arasaki, K;Tagaya, M
ZW 10是一种与动粒相关的动力素相互作用蛋白，已知其直接参与关闭纺锤体检查点。在本研究中，我们表明，ZW 10位于内质网以及在胞质溶胶中的间期，并形成一个亚复合物与RINT-1（Rad 50相互作用蛋白）和p31在一个大的复杂包括syntaxin 18，内质网本地化的t-陷阱牵连在膜贩运。与传统的突触融合蛋白结合蛋白ZW 10、RINT-1和p31一样。在NSF和α-SNAP存在下，Mg 2 + -ATP处理后，从突触融合蛋白18解离，而亚复合物未被分解。过表达，显微注射和敲低实验表明，ZW 10参与内质网和高尔基体之间的膜运输。本研究结果揭示了纺锤体检查点蛋白ZW 10在间期的一个意想不到的作用。
ZW10, a dynamitin-interacting protein associated with kinetochores, is known to participate directly in turning off of the spindle checkpoint. In the present study, we show that ZW10 is located in the endoplasmic reticulum as well as in the cytosol during interphase, and forms a subcomplex with RINT-1 (Rad50-interacting protein) and p31 in a large complex comprising syntaxin 18, an endoplasmic reticulum-localized t-SNARE implicated in membrane trafficking. Like conventional syntaxin-binding proteins, ZW10, RINT-1 and p31. dissociated from syntaxin 18 upon Mg2+ -ATP treatment in the presence of NSF and alpha-SNAP, whereas the subcomplex was not disassembled. Overexpression, microinjection and knockdown experiments revealed that ZW10 is involved in membrane trafficking between the endoplasmic reticulum and Golgi. The present results disclose an unexpected role for a spindle checkpoint protein, ZW10, during interphase.