Helium-neon laser irradiation stimulates cell proliferation through photostimulatory effects in mitochondria
Helium-neon laser irradiation stimulates cell proliferation through photostimulatory effects in mitochondria
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DOI：
10.1038/sj.jid.5700826
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发表时间：
2007-08-01
影响因子：
6.5
通讯作者：
Yu, Hsin-Su
中科院分区：
文献类型：
--
作者：
Hu, Wan-Ping;Wang, Jeh-Jeng;Yu, Hsin-Su
先前的研究表明，可见光（400- 700 nm）可能会影响细胞功能。最近的证据表明，细胞增殖可以通过氦氖激光（He-Ne激光，632.8nm）与线粒体光受体细胞色素c氧化酶的相互作用来触发。我们以前的研究表明，He-Ne辐射诱导黑色素瘤细胞系A2058细胞增殖增加，但不迁移。本研究的目的是探讨氦氖激光诱导的光刺激效应的机制。以A2058细胞为模型，研究了He-Ne激光诱导的光生物学效应。He-Ne辐射通过增强细胞色素c氧化酶活性和促进Jun N-末端激酶（JNK）/激活蛋白-1（AP-1）表达的磷酸化而立即诱导线粒体膜电位（Delta Psi（mt））、ATP和cAMP增加。He-Ne辐射诱导的A2058细胞增殖被添加Delta Psi（mt）和JNK抑制剂显著地废除。此外，用He-Ne激光处理导致对A2058细胞释放IL-8和转化生长因子β 1的延迟效应。这些结果表明，He-Ne辐射增强线粒体过程中的光刺激效应，这涉及JNK/AP-1激活和增强生长因子释放，并最终导致A2058细胞增殖。
Previous reports have shown that cellular functions could be influenced by visual light (400-700nm). Recent evidence indicates that cellular proliferation could be triggered by the interaction of a helium-neon laser (He-Ne laser, 632.8nm) with the mitochondrial photoacceptor-cytochrome c oxidase. Our previous studies demonstrated that He-Ne irradiation induced an increase in cell proliferation, but not migration, in the melanoma cell line A2058 cell. The aim of this study was to investigate the underlying mechanisms involved in photostimulatory effects induced by an He-Ne laser. Using the A2058 cell as a model for cell proliferation, the photobiologic effects induced by an He-Ne laser were studied. He-Ne irradiation immediately induced an increase in mitochondrial membrane potential (Delta Psi(mt)), ATP, and cAMP via enhanced cytochrome c oxidase activity and promoted phosphorylation of Jun N-terminal kinase (JNK)/activator protein-1 (AP-1) expressions. He-Ne irradiation-induced A2058 cell proliferation was significantly abrogated by the addition of Delta Psi(mt), and JNK inhibitors. Moreover, treatment with an He-Ne laser resulted in delayed effects on IL-8 and transforming growth factor-beta 1 release from A2058 cells. These results suggest that He-Ne irradiation elicits photostimulatory effects in mitochondria processes, which involve JNK/AP-1 activation and enhanced growth factor release, and ultimately lead to A2058 cell proliferation.