Comparative Ki-67 expression and apoptosis in the odontogenic keratocyst associated with or without an impacted tooth in addition to unilocular and multilocular varieties.
Comparative Ki-67 expression and apoptosis in the odontogenic keratocyst associated with or without an impacted tooth in addition to unilocular and multilocular varieties.
复制标题
除了单房和多房品种之外，有或没有受影响牙齿的牙源性角化囊肿中 Ki-67 表达和细胞凋亡的比较。
DOI：
10.3349/ymj.2003.44.5.841
复制
发表时间：
2003
影响因子：
2.4
通讯作者：
J. Yoon
中科院分区：
文献类型：
--
作者：
Do Kyung Kim;Sang‐Gun Ahn;Jin Kim;J. Yoon
目前尚不清楚牙源性角化囊肿（OKC）是否存在阻生牙或放射学类型会改变临床生物学行为和治疗方法。本研究评估了与埋伏牙相关或不相关的OKC的增殖活性和细胞凋亡的比较，以及单房和多房OKC品种之间的比较。采用TUNEL法检测32例OKC（有阻生牙的OKC， n=16；无阻生牙的OKC， n=16）和10例牙性囊肿（DC）的Ki-67增殖标志物的免疫组织化学表达及凋亡反应。与DC相比，OKC表现出更大的增殖潜能和更多的凋亡反应。特别是，OKC中增殖细胞和凋亡细胞分别主要位于基底上层和浅层。然而，在与阻生牙相关或不相关的OKC之间，以及在单房和多房OKC品种之间，在增殖活性或细胞凋亡方面没有发现显著差异。总之，OKC以细胞增殖和凋亡增加为特征，表明其具有独特的增殖和分化过程。人们认为，不完全切除或其他因素，而不是内在生长或凋亡，可能是多房性OKC侵袭性生物学行为或复发的主要原因。
It is not known whether the presence of an impacted tooth or the radiographic types in an odontogenic keratocyst (OKC) change the clinical biologic behavior and therapeutic approaches. This study evaluated the comparative proliferative activity and apoptosis in OKC associated with or without an impacted tooth, as well as between the unilocular and multilocular OKC varieties. Immunohistochemical expression of Ki-67 as a proliferation marker and the apoptotic reactions were assessed by the TUNEL method for 32 cases of OKC (OKC with impacted tooth, n=16; OKC without impacted tooth, n=16) and 10 cases of dentigerous cyst (DC). OKC showed a greater proliferative potential and more apoptotic reactions than DC. In particular, OKC contained proliferating and apoptotic cells situated predominantly in the suprabasal and superficial layers, respectively. However, no significant difference was found between OKC associated with or without impacted tooth, or between the unilocular and multilocular OKC varieties, in terms of proliferative activity or apoptosis. In conclusion, OKC is characterized by an increase in both cell proliferation and apoptosis, suggesting a unique proliferative and differentiation process. It is believed that incomplete removal or other contributing factors, rather than intrinsic growth or apoptosis, may be the main reasons for the aggressive biologic behavior or recurrence in multilocular OKC.