Drought tolerance established by enhanced expression of the CC-NBS-LRR gene, ADR1, requires salicylic acid, EDS1 and ABI1
Drought tolerance established by enhanced expression of the CC-NBS-LRR gene, ADR1, requires salicylic acid, EDS1 and ABI1
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DOI：
10.1111/j.1365-313x.2004.02086.x
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发表时间：
2004-06-01
期刊：
影响因子：
7.2
通讯作者：
Loake, GJ
中科院分区：
文献类型：
--
作者：
Chini, A;Grant, JJ;Loake, GJ
活化抗病性1（ADR 1）的活化标记等位基因先前已显示传递广谱抗病性。ADR 1编码一个卷曲螺旋（CC）-核苷酸结合位点（NBS）-富含亮氨酸重复序列（LRR）蛋白，与丝氨酸/苏氨酸蛋白激酶具有同源性。在这里，我们表明，无论是组成型或条件增强表达的ADR 1赋予显着的耐旱性。这不是防御相关突变体的一般特征，因为组成型表达系统获得抗性（SAR）的cir（组成型诱导抗性）1、cir 2和cpr（PR基因的组成型表达子）1未能表现出这种表型。adr 1植物的交叉耐受性不是一个特征，相反，它们表现出对温度和盐胁迫的敏感性增加。因此，adr 1激活的信号可能会拮抗一些应激反应。非生物标记基因的北方分析表明，脱水反应元件（DRE）B2 A，而不是DREB 1A，RD（响应脱水）29 A和RD 22在adr 1植物系中表达。此外，DREB 2A的表达是水杨酸（SA）依赖性的，但NPR（PR基因的非表达）1的独立性。在adr 1/ADR 1 nahG（naphthalene hydroxylase G）、adr 1/ADR 1 eds（enhanced disease susceptibility）1和adr 1/ADR 1 abi 1双突变体中，耐旱性显著降低。对含有条件性adr 1等位基因的植物的微阵列分析表明，大量上调的基因先前与脱水反应有关。因此，生物和非生物信号通路可能共享多个节点，并且它们的输出可能具有显著的功能重叠。
An activation-tagged allele of activated disease resistance 1 (ADR1) has previously been shown to convey broad spectrum disease resistance. ADR1 was found to encode a coiled-coil (CC)-nucleotide-binding site (NBS)-leucine-rich repeat (LRR) protein, which possessed domains of homology with serine/threonine protein kinases. Here, we show that either constitutive or conditional enhanced expression of ADR1 conferred significant drought tolerance. This was not a general feature of defence-related mutants because cir (constitutive induced resistance)1, cir2 and cpr (constitutive expressor of PR genes)1, which constitutively express systemic acquired resistance (SAR), failed to exhibit this phenotype. Cross-tolerance was not a characteristic of adr1 plants, rather they showed increased sensitivity to thermal and salinity stress. Hence, adr1-activated signalling may antagonise some stress responses. Northern analysis of abiotic marker genes revealed that dehydration-responsive element (DRE)B2A but not DREB1A, RD (response to dehydration)29A or RD22 was expressed in adr1 plant lines. Furthermore, DREB2A expression was salicylic acid (SA) dependent but NPR (non-expressor of PR genes)1 independent. In adr1/ADR1 nahG (naphthalene hydroxylase G), adr1/ADR1 eds (enhanced disease susceptibility)1 and adr1/ADR1 abi1 double mutants, drought tolerance was significantly reduced. Microarray analyses of plants containing a conditional adr1 allele demonstrated that a significant number of the upregulated genes had been previously implicated in responses to dehydration. Therefore, biotic and abiotic signalling pathways may share multiple nodes and their outputs may have significant functional overlap.