Neuropharmacologic effects of vibration on the dorsal root ganglion. An animal model.
Neuropharmacologic effects of vibration on the dorsal root ganglion. An animal model.
复制标题
振动对背根神经节的神经药理学作用。
DOI：
10.1097/00007632-198805000-00015
复制
发表时间：
1988
期刊：
影响因子：
3
通讯作者：
Seroussi,R
中科院分区：
文献类型：
--
作者：
Weinstein,J;Pope,M;Schmidt,R;Seroussi,R
低频振动对背根神经节的神经药理学影响，是下背痛的一个流行病学原因，最近才被描述。本研究旨在验证已知在背根神经节细胞体中产生的P物质和VIP将受到低频振动影响的假设。三只新西兰白色兔子以离散频率（2-10 Hz）振动以确定兔子脊柱的共振频率。共振频率在（3.5-5.0 Hz）范围内。峰值振幅为4.5 Hz。然后将10只雌性新西兰白色家兔配对成两组，每组5只。一组作为对照组，除了振动之外，进行与实验组完全相同的程序。取双侧L4-5和L5-6背根神经节，用放射免疫法提取P物质和VIP。对照组P物质平均值为14.06pg/ml，实验组和振动组P物质平均值为8.40pg/ml（P< 0.003）。对照组家兔免疫反应性血管活性肠肽平均值为9.58pg/ml，而实验组和振动组家兔免疫反应性血管活性肠肽平均值为20.9pg/ml，P< 0.07。P物质是背根神经节中唯一参与伤害性感受器传递的神经肽。VIP是一种神经肽，在损伤后的神经系统重组中发挥作用。低频振动对背根神经节递质的影响对于理解振动是背痛的原因是必不可少的。低频振动后观察到的P物质局部减少和VIP增加与外周损伤后的结果一致。VIP的升高可能参与了周围神经损伤后神经和脊髓背角的病理生理过程。这些变化与我们以前的工作是一致的，并提供了一个机会，探讨与这些发现相关的功能异常。
The neuropharmacologic effects of low frequency vibration on the dorsal root ganglion, a reported epidemiological cause of low-back pain, have only recently been described. This Investigation was undertaken to validate the hypothesis that substance P and VIP, known to be produced in the dorsal root ganglion cell bodies, will be affected by low frequency vibration. Three New Zealand white rabbits were vibrated at discrete frequencies (2–10 Hz) to determine the resonant frequency of the rabbit spine. The resonating frequency was in the (3.5–5.0 Hz) range. The peak amplitude was at 4.5 Hz. Ten female New Zealand white rabbits were then paired into two groups of five. One group served as a control and had exactly the same procedures performed as the experimental group except for the vibration. The L4–5 and L5–6 dorsal root ganglia were removed bilaterally and prepared for substance P and VIP extraction by radioimmunpassay technique. The control rabbits mean immunoreactive substance P was 14.06 pg/ml tissue, whereas the experimental or vibrated rabbits had a mean of 8.40 pg/ml (P< 0.003). The control rabbits mean immunoreactive vasoactive Intestinal peptide was 9.58 pg/ml whereas the experimental or vibrated rabbits had a mean of 20.9 pg/ml, P< 0.07. Substance P is only one of several dorsal root ganglion neuropeptides that may play a role in nociceptor transmisssion. VIP is a neuropeptide that plays a role in reorganization of the nervous system following Injury. The effects of low frequency vibration on dorsal root ganglion transmitters are essential to the understanding of vibration as a cause of back pain. The localized decrease in substance P and increased VIP seen following low frequency vibration are compatible with results following peripheral Injury. The Increase in VIP may play a role In the pathophysiology processes in nerve and dorsal spinal cord following peripheral nerve Injury. The changes are compatible with our previous work and offer an opportunity to explore functional abnormalities associated with these findings.