AMPLIFICATION OF AN ESTERASE GENE IS RESPONSIBLE FOR INSECTICIDE RESISTANCE IN A CALIFORNIA CULEX MOSQUITO
AMPLIFICATION OF AN ESTERASE GENE IS RESPONSIBLE FOR INSECTICIDE RESISTANCE IN A CALIFORNIA CULEX MOSQUITO
复制标题
DOI：
10.1126/science.3755546
复制
发表时间：
1986-08-15
期刊：
影响因子：
56.9
通讯作者：
GEORGHIOU, GP
中科院分区：
文献类型：
--
作者：
MOUCHES, C;PASTEUR, N;GEORGHIOU, GP
将致倦库蚊（Culex quinquefasciatus）中负责对多种有机磷（OP）杀虫剂的抗性的酯酶基因克隆到噬菌体Gt 11中。利用该基因研究了致倦库蚊（CulexquinquefasciatusSay，Tem-R株）酯酶B1高产的遗传机制。Tem-R菌株的成年人被发现拥有比敏感菌株（S-Lab）的成年人多至少250倍的基因拷贝。杀虫剂的选择可能导致整个正常发育的繁殖昆虫中编码解毒酶的基因扩增，这一发现强调了这种机制的生物学重要性，并开辟了杀虫剂抗性管理的新研究领域。
An esterase gene from the mosquito Culex quinquefasciatus that is responsible for resistance to a variety of organophosphorus (OP) insecticides was cloned in .lambda.gt11 phage. Ths gene was used to investigate the genetic mechanism of the high production of the esterase B1 it encodes in OP-resistant Culex quinquefasciatus Say (Tem-R strain) from California. Adults of the Tem-R strain were found to possess at least 250 times more copies of the gene than adults of a susceptible strain (S-Lab). The finding that selection by pesticides may result in the amplification of genes encoding detoxifying enzymes in whole, normally developed, reproducing insects emphasizes the biological importance of this mechanism and opens new areas of investigation in pesticide resistance management.