Serum Exosomes Attenuate H2O2-Induced Apoptosis in Rat H9C2 Cardiomyocytes via ERK1/2
Serum Exosomes Attenuate H2O2-Induced Apoptosis in Rat H9C2 Cardiomyocytes via ERK1/2
复制标题
血清外泌体通过 ERK1/2 减弱 H2O2 诱导的大鼠 H9C2 心肌细胞凋亡
DOI：
10.1007/s12265-018-9791-3
复制
发表时间：
2019-02-01
影响因子：
3.4
通讯作者：
Zhou, Lei
中科院分区：
文献类型：
--
作者：
Li, Pengfei;Liu, Zhuyuan;Zhou, Lei
外泌体是可以从细胞释放到血清中的小尺寸囊泡。外泌体在调节细胞增殖、凋亡、细胞周期和代谢等许多生物学过程中发挥重要作用。然而，血浆外泌体在大鼠H9 C2心肌细胞凋亡中的作用和机制在很大程度上是未知的。在这项研究中，我们分离了血浆外泌体，如标记蛋白CD 63所证实的。使用流式细胞术和蛋白质印迹分析，我们发现，外泌体衰减过氧化氢（H2 O2）诱导的细胞凋亡，提高大鼠H9 C2心肌细胞的存活率。此外，血清exosomes对H9 C2心肌细胞的抗凋亡作用可能是通过激活ERK 1/2信号通路而实现的。这些数据表明，血浆exosomes对心肌细胞凋亡具有保护作用，可能成为心肌损伤的一种新的治疗策略。
Exosomes are small-sized vesicles that can be released from cells into the serum. Exosomes play important roles in regulating many biological processes including cell proliferation, apoptosis, cell cycle, and metabolism. However, the roles and mechanisms of plasma exosomes in the apoptosis of rat H9C2 cardiomyocytes are largely unknown. In this study, we isolated plasma exosomes as confirmed by the marker protein CD63. Using flow cytometry and western blot analysis, we found that exosomes attenuated hydrogen peroxide (H2O2)-induced apoptosis and improved survival of rat H9C2 cardiomyocytes. Furthermore, the anti-apoptosis effects of serum exosomes in rat H9C2 cardiomyocytes were mediated by the activation of ERK1/2 signaling pathway. These data indicated that plasma exosomes had the protective effects against cardiomyocyte apoptosis and might be a novel therapy strategy for myocardial injury.