Chemically Reactive Supramolecular Hydrogel Coupled with a Signal Amplification System for Enhanced Analyte Sensitivity
Chemically Reactive Supramolecular Hydrogel Coupled with a Signal Amplification System for Enhanced Analyte Sensitivity
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DOI：
10.1021/ja5131534
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发表时间：
2015-03-11
影响因子：
15
通讯作者：
Hamachi, Itaru
中科院分区：
文献类型：
--
作者：
Yoshii, Tatsuyuki;Onogi, Shoji;Hamachi, Itaru
多组分超分子水凝胶被构建用于小分子生物标志物的灵敏、裸眼检测。一个树枝状的自分解分子和相应的酶作为信号放大系统，稳定地嵌入过氧化氢（H2 O2）响应的超分子水凝胶（BPmoc-F-3），与其他酶。在不存在靶生物标志物的情况下，掺入这些多种组分基本上不会降低杂化BPmoc-F-3凝胶的纳米结构和机械强度，但是可以通过在凝胶基质内组合操作的多种酶促和化学级联反应添加生物标志物来破坏。通过凝胶-溶胶转变检测的生物标志物如H2 O2、葡萄糖和尿酸的灵敏度通过信号放大系统显著增强。由这些多组分水凝胶组成的阵列芯片能够检测人血浆样品中的高尿酸血症疾病水平。
Multicomponent supramolecular hydrogels are constructed for sensitive, naked-eye detection of small-molecule biomarkers. A dendritic self-immolative molecule and the corresponding enzyme as a signal amplification system were stably embedded in a hydrogen peroxide (H2O2)-responsive supramolecular hydrogel (BPmoc-F-3), together with other enzymes. The nanostructure and mechanical strength of the hybrid BPmoc-F-3 gel were not substantially diminished by incorporation of these multiple components in the absence of target biomarkers, but could be destroyed by addition of the biomarker through the multiple enzymatic and chemical cascade reactions operating in combination within the gel matrix. The sensitivity to biomarkers such as H2O2, glucose, and uric acid, detected by gel-sol transition, was significantly enhanced by the signal amplification system. An array chip consisting of these multicomponent hydrogels enabled the detection of the level of hyperuricemia disease in human plasma samples.