The Expression of SIRT6 Is Associated With Treatment Outcome in Elder Patients With Oral Cancer
The Expression of SIRT6 Is Associated With Treatment Outcome in Elder Patients With Oral Cancer
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DOI：
10.21873/anticanres.15872
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发表时间：
2022-07
影响因子：
2
通讯作者：
Haruka Yoshii;K. Sekihara;Yuka Ideta;Shintaro Nakajima;I. Kato;M. Okubo-Sato;Kei Sugiura;K. Mitsudo;M. Kioi
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文献类型：
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作者：
Haruka Yoshii;K. Sekihara;Yuka Ideta;Shintaro Nakajima;I. Kato;M. Okubo-Sato;Kei Sugiura;K. Mitsudo;M. Kioi
背景/目的：SIRT6是人类七个sirtuin基因之一，已知在结直肠癌和卵巢癌中作为抑癌基因，尽管它在其他癌症中上调。因此，SIRT6被认为同时发挥肿瘤抑制和促进作用。然而，SIRT6与口腔鳞状细胞癌（OSCC）的关系及其在OSCC发病机制中的作用目前尚不清楚。本研究旨在探讨SIRT6在OSCC患者中的表达及其作为早期检测和预后预测的生物标志物的潜力。材料和方法：采用免疫组织化学、实时定量RT-PCR和微阵列分析方法检测OSCC临床标本中SIRT6的表达及其与临床病理特征的关系。结果：SIRT6 mRNA和蛋白在OSCC组织中的表达水平高于非癌组织（p<0.05）。SIRT6表达在年龄≥65岁的患者中占主导地位，并与较短的总生存期显著相关。在微阵列分析中，一些sirt6相关基因，如ANXA2，在OSCC中上调。结论：SIRT6在OSCC中参与肿瘤稳态，导致预后不良。SIRT6可能不仅是治疗的新靶点，也是OSCC的预后标志物。
Background/Aim: SIRT6 is one of seven human sirtuin genes and is known to act as an onco-suppressor gene in colorectal and ovarian cancers, although it is up-regulated in other cancers. Thus, SIRT6 is considered performing both tumor-suppressing and promoting roles. However, the association of SIRT6 with oral squamous cell carcinoma (OSCC) and its role in OSCC pathogenesis is currently unclear. This study aimed to investigate the expression of SIRT6 in patients with OSCC and its potential as a biomarker for early detection and prognosis prediction. Materials and Methods: Immunohistochemistry, quantitative real-time RT-PCR, and microarray analyses were performed to determine SIRT6 expression and its association with clinicopathological features in OSCC using clinical specimens. Results: SIRT6 mRNA and protein expression levels were higher in OSCC tissues than in noncancerous tissues (p<0.05). SIRT6 expression was predominant in patients aged ≥65 years and significantly correlated with shorter overall survival. In the microarray analysis, some SIRT6-associated genes, such as ANXA2, were up-regulated in OSCC. Conclusion: SIRT6 plays a role in tumor homeostasis, leading to a poor prognosis in OSCC. SIRT6 may represent a novel target not only for treatment, but also as a prognostic marker in OSCC.