Effects of the phospholipase inhibitor mepacrine on injury in ischemic and metabolically inhibited adult isolated myocytes.
Effects of the phospholipase inhibitor mepacrine on injury in ischemic and metabolically inhibited adult isolated myocytes.
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磷脂酶抑制剂 mepacrine 对缺血和代谢受抑制的成年离体肌细胞损伤的影响。
DOI：
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发表时间：
1991
影响因子：
6
通讯作者：
C. Ganote
中科院分区：
文献类型：
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作者：
S. Armstrong;C. Ganote
磷脂酶抑制剂 mepacrine 已被证明可以延迟代谢受抑制的培养心肌细胞的细胞死亡。本研究的目的是确定mepacrine是否也能延迟代谢抑制和缺血成年大鼠心肌细胞的细胞死亡和渗透脆性的发展。将分离的肌细胞悬浮液与 3 mmol/l（毫摩尔）碘乙酸和 6 mmol/l amytal（抑制）一起孵育，或者在存在和不存在 10 或 50 mumol/l（微摩尔）mepacrine 的情况下沉淀成浆液并用油分层（缺血）。监测挛缩率、通过台盼蓝渗透性测定的细胞活力、在170 mOsm培养基中渗透膨胀后的细胞活力（渗透脆性）以及细胞形态。 Mepacrine 对挛缩率没有影响，但发现在代谢抑制细胞和缺血细胞等渗培养期间显着延迟细胞死亡。相反，mepacrine 对渗透脆性的发展没有影响。在无钙培养基中孵育代谢受到抑制的肌细胞不会延迟挛缩或细胞损伤，但会减弱mepacrine的保护作用。这项研究证实了先前的报道，即mepacrine可以保护细胞免受损伤，将保护观察范围扩大到缺血性分离的成体肌细胞，但表明mepacrine不会抑制渗透脆性的发展。
The phospholipase inhibitor mepacrine has been shown to delay cell death of metabolically inhibited cultured cardiomyocytes. The present study was initiated to determine if mepacrine also delays cell death and development of osmotic fragility of both metabolically inhibited and ischemic adult rat cardiomyocytes. Isolated myocyte suspensions were incubated with 3 mmol/l (millimolar) iodoacetic acid and 6 mmol/l amytal (inhibited) or were pelleted into a slurry and layered with oil (ischemic) in the presence and absence of 10 or 50 mumol/l (micromolar) mepacrine. Rates of contracture, cell viability as determined by trypan blue permeability, cell viability after osmotic swelling in 170 mOsm media (osmotic fragility), and cell morphology were monitored. Mepacrine had no effects on rates of contracture, but was found to significantly delay cell death during isotonic incubations of both metabolically inhibited and ischemic cells. In contrast, mepacrine had no effect on the development of osmotic fragility. Incubation of metabolically inhibited myocytes in calcium-free media did not delay contracture or cell injury, but did attenuate the protective effects of mepacrine. This study confirms previous reports that mepacrine protects cells from injury, extends the observations of protection to ischemic isolated adult myocytes, but shows that development of osmotic fragility is not inhibited by mepacrine.