Platelets Express Activated P2Y12 Receptor in Patients With Diabetes Mellitus
Platelets Express Activated P2Y12 Receptor in Patients With Diabetes Mellitus
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糖尿病患者的血小板表达激活的 P2Y(12) 受体
DOI：
10.1161/circulationaha.116.026995
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发表时间：
2017-08-29
期刊：
影响因子：
37.8
通讯作者：
Ding, Zhongren
中科院分区：
文献类型：
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作者：
Hu, Liang;Chang, Lin;Ding, Zhongren
背景：糖尿病患者的血小板活性过高。过度活化的血小板可能导致心血管并发症，并且在糖尿病环境下对抗血小板药物反应不佳。然而，血小板过度活化的潜在机制尚未完全明确。
方法：我们测量了2型糖尿病患者血小板以及糖尿病大鼠血小板上P2Y(12)的表达。我们还通过测量环磷腺苷（cAMP）和血管舒张剂刺激磷蛋白（VASP）的磷酸化来测定血小板P2Y(12)的活化情况。在大鼠中比较了AR - C78511和坎格瑞洛的抗血小板和抗血栓作用。最后，我们探讨了核因子 - κB通路在调节巨核细胞中P2Y(12)受体表达的作用。
结果：与健康受试者相比，2型糖尿病患者的血小板P2Y(12)水平高4倍。P2Y(12)表达与二磷酸腺苷（ADP）诱导的血小板聚集相关（r = 0.89，P<...（此处原文似乎不完整）
BACKGROUND: Platelets from patients with diabetes mellitus are hyperactive. Hyperactivated platelets may contribute to cardiovascular complications and inadequate responses to antiplatelet agents in the setting of diabetes mellitus. However, the underlying mechanism of hyperactivated platelets is not completely understood.METHODS: We measured P2Y(12) expression on platelets from patients with type 2 diabetes mellitus and on platelets from rats with diabetes mellitus. We also assayed platelet P2Y(12) activation by measuring cAMP and VASP phosphorylation. The antiplatelet and antithrombotic effects of AR-C78511 and cangrelor were compared in rats. Finally, we explored the role of the nuclear factor-kappa B pathway in regulating P2Y(12) receptor expression in megakaryocytes.RESULTS: Platelet P2Y(12) levels are 4-fold higher in patients with type 2 diabetes mellitus compared with healthy subjects. P2Y(12) expression correlates with ADP-induced platelet aggregation (r=0.89, P