Variations in glycosylation of !von Willebrand factor with O-linked sialylated T antigen are associated with its plasma levels
Variations in glycosylation of !von Willebrand factor with O-linked sialylated T antigen are associated with its plasma levels
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DOI：
10.1182/blood-2006-06-032706
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发表时间：
2007-03-15
期刊：
影响因子：
20.3
通讯作者：
Lenting, Peter J.
中科院分区：
文献类型：
--
作者：
van Schooten, Carina J. M.;Denis, Cecile V.;Lenting, Peter J.
已知von Willebrand因子(VWF)的糖基化特征强烈影响其血浆水平。VWF含有几种碳水化合物结构，包括主要由唾液酸化T抗原(NeuAc(Alpha2-3)Gal-(beta 1-3)-[NeuAc(Alpha2-6)]GaINAc)组成的O-连接糖链。目前尚不清楚O-连接的碳水化合物是否会影响VWF水平。我们建立了一种基于神经氨酸酶孵育的免疫吸附试验，允许花生凝集素(PNA)随后与VWF上期望分析的O-连接T抗原结合。在健康人中，PNA结合率与VWF抗原水平呈负相关(n=111；Pearson等级=-0.43；P
The glycosylation profile of von Willebrand factor (VWF) is known to strongly influence its plasma levels. VWF contains several carbohydrate structures, including O-linked glycans that primarily consist of sialylated T antigen (NeuAc(alpha 2-3)Gal-(beta 1-3)-[NeuAc(alpha 2-6)]GaINAc). It is not yet known whether O-linked carbohydrates affect VWF levels. We developed an immunosorbent assay based on neuraminidase incubation allowing subsequent binding of peanut agglutinin (PNA) to desialylated O-linked T antigen on VWF. An inverse relation was found between PNA binding and VWF antigen levels in healthy individuals (n = 111; Pearson rank = -0.43; P