Localized single-stranded bubble mechanism for cyclization of short double helix DNA
Localized single-stranded bubble mechanism for cyclization of short double helix DNA
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DOI：
10.1103/physrevlett.93.108108
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发表时间：
2004-09-03
影响因子：
8.6
通讯作者：
Marko, JF
中科院分区：
文献类型：
--
作者：
Yan, J;Marko, JF
最近的实验表明，长度约为100个碱基对的双链DNA分子的环化几率比基于已知的双螺旋弯曲模数预期的环化几率大10(5)倍。我们认为，对于短分子，几个碱基对的单链DNA的形成可以提供一个“灵活的铰链”，促进环的形成。详细的计算表明，这一机制解释了实验数据。
Recent experiments indicate that double-stranded DNA molecules of approximately 100 base pairs in length have a probability of cyclization which is up to 10(5) times larger than that expected based on the known bending modulus of the double-helix. We argue that for short molecules, the formation of a few base pairs of single-stranded DNA can provide a "flexible hinge" that facilitates loop formation. A detailed calculation shows that this mechanism explains the experimental data.