Comparison of hippocampal synaptosome proteins in young-adult and aged rats
Comparison of hippocampal synaptosome proteins in young-adult and aged rats
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DOI：
10.1016/j.neulet.2005.02.053
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发表时间：
2005-07-01
影响因子：
2.5
通讯作者：
Endo, T
中科院分区：
文献类型：
--
作者：
Sato, Y;Yamanaka, H;Endo, T
海马体在学习和记忆功能中很重要，但它帮助学习和记忆功能的能力随着年龄的增长而下降。在这项研究中，我们检测了在衰老过程中表达变化的海马蛋白。用双向荧光差示凝胶电泳法对幼年(9周龄)大鼠和老年(30月龄)大鼠海马区突触体蛋白进行比较，发现在幼年大鼠和老年大鼠的约1000个点中，有24个点的表达存在差异。在这24个斑点中，有19个用肽质量指纹图谱进行了鉴定。这些蛋白质包括伴侣蛋白和与细胞骨架、神经传递、信号转导和能量供应相关的蛋白。与细胞骨架相关的蛋白质包括肌动蛋白和T-复合体1，后者被认为在肌动蛋白折叠中发挥作用。在老年大鼠突触中，肌动蛋白表达上调，而T-复合体1表达下调。这些结果表明，肌动蛋白细丝形成的年龄依赖性变化与衰老相关的神经元功能障碍有关。(C)2005爱思唯尔爱尔兰有限公司。保留所有权利。
The hippocampus is important in learning and memory functions but its ability to aid in these functions declines during aging. In this study, we examined hippocampal proteins whose expressions changed in the aging process. A comparison of synaptosome proteins of hippocampus prepared from youno-adult (9-week-old) rats with those from aged (30-month-old) rats by two-dimensional fluorescence difference gel electrophoresis revealed 24 spots that were expressed differently among about 1000 spots detected in both young-adult and aged rat samples. Nineteen of these 24 spots were identified by peptide mass fingerprinting. These proteins included chaperone proteins and proteins related to the cytoskeleton, neurotransmission, signal transduction and energy supply. The cytoskeleton-related proteins included actin and T-complex 1, which is thought to play a role in actin folding. Actin was up-regulated but T-complex 1 was down-regulated in aged rat synapses. These results suggest that age-dependent changes of actin filament formation are related to neuronal dysfunction associated with aging. (c) 2005 Elsevier Ireland Ltd. All rights reserved.