Electron microscopic detection of statherin in secretory granules of human major salivary glands
Electron microscopic detection of statherin in secretory granules of human major salivary glands
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电镜检测人大唾液腺分泌颗粒中富酪素
DOI：
10.1111/j.1469-7580.2008.00888.x
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发表时间：
2008
影响因子：
2.4
通讯作者：
A. Riva
中科院分区：
文献类型：
--
作者：
M. Isola;T. Cabras;R. Inzitari;M. S. Lantini;E. Proto;M. Cossu;A. Riva
为了增加目前关于唾液酸甘油酶分泌到口腔中的知识，通过使用包埋后免疫金染色技术研究了该肽在人唾液腺中的超微结构定位。在腮腺和下颌下腺的浆液细胞颗粒内发现了Statherin反应。在腮腺颗粒中，免疫染色优先存在于电子密度较低的区域，而在下颌下浆液颗粒中，反应性是均匀的，致密核心总是染色。相比之下，在主要舌下腺的浆液细胞中没有观察到或观察到弱反应。这些研究结果揭示了第一次通过电子透射显微镜的唾液酸甘油酶的亚细胞定位，并证实了三种主要类型的唾液腺，腮腺和下颌下腺是唾液酸甘油酶的最大来源。此外，他们建议，一个以上的包装机制可能涉及在唾液腺浆液颗粒内的staterin的存储。
In order to increase current knowledge regarding statherin secretion into the oral cavity, ultrastructural localization of this peptide was investigated in human salivary glands by using a post‐embedding immunogold staining technique. Statherin reactivity was found inside the granules of serous cells of parotid and submandibular glands. In parotid granules immunostaining was preferentially present in the less electron‐dense region, whereas in submandibular serous granules the reactivity was uniform and the dense core always stained. By contrast, none or weak reactivity was observed in serous cells of major sublingual glands. These findings reveal for the first time the subcellular localization of statherin by electron transmission microscopy and confirm that of the three major types of salivary glands, the parotid and submandibular glands are the greatest source of salivary statherin. Moreover, they suggest that more than one packaging mechanism may be involved in the storage of statherin within serous granules of salivary glands.
唾液蛋白质的分类和分泌。
DOI：
10.1177/10454411930040031901
发表时间：
1993
期刊：
Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists
影响因子：
--
作者：
Castle,JD;Castle,AM
通讯作者：
Castle,AM
唾液蛋白在人腮腺和下颌下腺泡细胞颗粒中的定位。
DOI：
10.1177/10454411930040032001
发表时间：
1993
期刊：
Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists
影响因子：
--
作者：
Takano,K;Malamud,D;Bennick,A;Oppenheim,F;Hand,AR
通讯作者：
Hand,AR