Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii.
Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii.
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蘑菇恩格氏酵母培养物中两种新单端孢霉烯族化合物和一种新铜烷型倍半萜化合物的鉴定及其细胞毒活性
DOI：
10.1007/s13659-014-0051-1
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发表时间：
2015-01-30
影响因子：
4.7
通讯作者：
Liu JK
中科院分区：
文献类型：
--
作者：
Wang Y;Zhang L;Li GT;Li ZH;Dong ZJ;Li Y;Liu JK
戈茨肉球菌（Engleromyces goetzii）是一种传统药用真菌，在中国西藏、四川和云南等地广泛用于治疗感染、炎症和癌症。从戈茨肉球菌的培养物中分离得到了两种新的单端孢霉烯类化合物，戈茨肉球菌酮A和B（1和2），一种新的柏木烷型倍半萜类化合物，命名为肉球菌素F（11），八种已知的单端孢霉烯类似物，接骨木醇（3）、3 - 脱氧接骨木醇（4）、单端孢霉烯酮（5）、木霉菌醇（6）、8 - 脱氧单端孢菌素（7）、单端孢菌素（8）、单端孢菌醇B（9）和单端孢菌醇A（10），以及一种已知的环戊烷型倍半萜类化合物环罗汉松二醇（12）。通过光谱分析对新化合物进行了结构解析。采用MTT法检测了单端孢霉烯类化合物1 - 10对人白血病细胞HL - 60、人肝癌细胞SMMC - 7721、人肺癌细胞A549、人乳腺癌细胞MCF - 7和人结肠癌细胞SW - 480的抗癌作用。单端孢菌醇A（10）对MCF - 7细胞的生长有显著抑制作用，IC50值为0.006 μM，与阳性对照紫杉醇的细胞毒活性相当，表明单端孢菌醇A（10）是一种潜在的抗癌药物。
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AbstractEngleromyces goetziiis a traditional medicinal mushroom that is widely used to treat infection, inflammation and cancer in Tibet, Sichuan and Yunnan provinces of China. Two new trichothecenes, engleromycones A and B (1and2), one new cuparane-type sesquiterpenoid named infuscol F (11), eight known trichothecene analogs, sambucinol (3), 3-deoxysambucinol (4), trichothecolone (5), trichodermol (6), 8-deoxytrichothecin (7), trichothecin (8), trichothecinol B (9) and trichothecinol A (10), and one known cyclopentanoid sesquiterpene cyclonerodiol (12) were isolated from the cultures ofE. goetzii. The new compounds were elucidated through spectroscopic analyses. The anticancer effects of trichothecenes1–10were examined in the HL-60, SMMC-7721, A549, MCF-7, and SW-480 human cancer cell lines using an MTT assay. Trichothecinol A (10) significantly inhibited the growth of MCF-7 cells, with an IC50value of 0.006 µM, which was comparable to the cytotoxic activity of the positive control, paclitaxel, indicating that trichothecinol A (10) represents a potential anticancer agent.Graphical Abstract