Critical role of CD81 in cognate T-B cell interactions leading to Th2 responses
Critical role of CD81 in cognate T-B cell interactions leading to Th2 responses
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DOI：
10.1093/intimm/14.5.513
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发表时间：
2002-05-01
影响因子：
4.4
通讯作者：
Levy, S
中科院分区：
文献类型：
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作者：
Deng, J;Dekruyff, RH;Levy, S
我们以前证明，CD 81（-/-）小鼠不能发展T（h）2偏向的免疫反应和过敏原诱导的气道高反应性。由于CD 81在活化的T细胞和B细胞上都有表达，我们研究了每种细胞类型的CD 81表达的作用。我们建立了一个体外系统，通过回交的CD 81缺失TCR转基因（Tg）小鼠和BCR Tg小鼠。在这里，我们证明，CD 81表达的T细胞是至关重要的诱导IL-4合成的B细胞。与CD 81（+/+）TCR Tg T细胞相比，CD 81（-/-）TCR Tg T细胞在IL-4产生方面受损，而CD 81（-/-）和CD 81（+/+）BCR Tg B细胞在CD 81（+/+）TCR Tg T细胞中诱导等量的IL-4。当被抗原呈递B细胞激活时，CD 81（-/-）TCR Tg T细胞表达降低水平的ICOS、加塔-3、STAT 6和磷酸化STAT 6。总之，这些结果表明，T细胞的CD 81表达大大增强了同源T-B细胞相互作用，并大大增强了导致T（h）2极化的细胞内活化途径。
We previously demonstrated that CD81(-/-) mice fail to develop T(h)2-biased immune responses and allergen-induced airway hyper-reactivity. Because CD81 is expressed on both activated T and on B cells, we examined the role of CD81 expression by each cell type. We established an in vitro system by backcrossing the CD81 deletion to TCR transgenic (Tg) mice and to BCR Tg mice. Here we demonstrate that CD81 expression by T cells is critical for their induction of IL-4 synthesis by B cells. CD81(-/-) TCR Tg T cells were impaired in IL-4 production compared to CD81(+/+) TCR Tg T cells, whereas CD81(-/-) and CD81(+/+) BCR Tg B cells induced equivalent amounts of IL-4 in CD81(+/+) TCR Tg T cells. CD81(-/-) TCR Tg T cells expressed reduced levels of ICOS, GATA-3, STAT6 and phosphorylated STAT6 when activated by antigen-presenting B cells. Taken together, these results indicate that CD81 expression by T cells greatly enhances cognate T-B cell interactions and greatly augments intracellular activation pathways leading to T(h)2 polarization.