Respiratory disease in rhesus macaques inoculated with SARS-CoV-2
Respiratory disease in rhesus macaques inoculated with SARS-CoV-2
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DOI：
10.1038/s41586-020-2324-7
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发表时间：
2020-05-12
期刊：
影响因子：
64.8
通讯作者：
de Wit, Emmie
中科院分区：
文献类型：
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作者：
Munster, Vincent J.;Feldmann, Friederike;de Wit, Emmie
2019 年冠状病毒病 (COVID-19) 是由一种新型冠状病毒（名为 SARS-CoV-2）引起的，病死率约为 2%，于 2019 年 12 月在中国武汉爆发(1,2)。在史无前例的全球传播(3)之后，世界卫生组织于 2020 年 3 月 11 日宣布 COVID-19 为大流行病。尽管有关人类 COVID-19 的数据正在稳步涌现，但 SARS-CoV-2 发病机制的某些方面只能在动物模型中进行详细研究，在动物模型中可以重复采样和组织收集。在这里，我们发现 SARS-CoV-2 会导致恒河猴出现持续 8 至 16 天的呼吸道疾病。肺部浸润是人类感染 COVID-19 的一个标志，在肺部 X 光照片中可以看到。我们在所有猕猴的鼻子和喉咙的拭子以及支气管肺泡灌洗液中检测到高病毒载量；在一只猕猴中，我们观察到直肠脱落时间较长。总之，恒河猴再现了大多数人类 COVID-19 病例中观察到的中度疾病。建立恒河猴作为 COVID-19 模型将增加我们对该疾病发病机制的了解，并有助于制定和测试医疗对策。恒河猴感染 SARS-CoV-2 会引起呼吸道疾病，在人类中重现了 COVID-19 的各个方面，因此将该物种建立为研究 SARS-CoV-2 发病机制的动物模型。
An outbreak of coronavirus disease 2019 (COVID-19), which is caused by a novel coronavirus (named SARS-CoV-2) and has a case fatality rate of approximately 2%, started in Wuhan (China) in December 2019(1,2). Following an unprecedented global spread(3), the World Health Organization declared COVID-19 a pandemic on 11 March 2020. Although data on COVID-19 in humans are emerging at a steady pace, some aspects of the pathogenesis of SARS-CoV-2 can be studied in detail only in animal models, in which repeated sampling and tissue collection is possible. Here we show that SARS-CoV-2 causes a respiratory disease in rhesus macaques that lasts between 8 and 16 days. Pulmonary infiltrates, which are a hallmark of COVID-19 in humans, were visible in lung radiographs. We detected high viral loads in swabs from the nose and throat of all of the macaques, as well as in bronchoalveolar lavages; in one macaque, we observed prolonged rectal shedding. Together, the rhesus macaque recapitulates the moderate disease that has been observed in the majority of human cases of COVID-19. The establishment of the rhesus macaque as a model of COVID-19 will increase our understanding of the pathogenesis of this disease, and aid in the development and testing of medical countermeasures.Infection with SARS-CoV-2 in rhesus macaques causes a respiratory disease that recapitulates aspects of COVID-19 in humans, establishing this species as an animal model for investigating the pathogenesis of SARS-CoV-2.