Three GnRH receptor types in laser-captured single cells of the cichlid pituitary display cellular and functional heterogeneity.
Three GnRH receptor types in laser-captured single cells of the cichlid pituitary display cellular and functional heterogeneity.
复制标题
DOI：
10.1073/pnas.0409494102
复制
发表时间：
2005-02
影响因子：
11.1
通讯作者：
I. Parhar;S. Ogawa;Y. Sakuma
中科院分区：
文献类型：
--
作者：
I. Parhar;S. Ogawa;Y. Sakuma
多种促性腺激素释放激素受体（GnRH-R）类型在调节促性腺激素和非促性腺激素细胞中的作用仍然是推测性的。为了解决这个问题，我们开发了一种技术，结合激光捕获显微切割的单个地高辛标记的垂体细胞，再加上实时定量PCR，以检查三种内源性GnRH-R类型（R1，R2和R3）的表达谱在未成熟和成熟的雄性罗非鱼Oreochromis niloticus。在这里，除了促性腺激素（促黄体生成素和促卵泡激素，FSH），我们还显示GnRH-Rs也存在于催乳素，促生长素，促甲状腺素，促黑素细胞（促黑素细胞激素，MSH），促肾上腺皮质激素和体肌动蛋白细胞。垂体细胞亚群表达单个（42.9%）、多个（32.4%）或缺乏（24.7%）GnRH-Rs。在未成熟男性中，含有GnRH-Rs组合的FSH细胞百分比显著高于成熟男性（R1+R2：24%，P <0.05; R1+R2+R3：25%，P <0.01），而仅显示R1和R1和R3转录本的百分比在成熟男性中更高（P <0.05）。R1和R3转录本在未成熟和成熟男性的MSH细胞中的拷贝数分别显著增加（P <0.05）。成熟雄性的其他垂体细胞（催乳素细胞，R1和R2;生长激素肌动蛋白细胞/促甲状腺素细胞/促肾上腺皮质素细胞，R1、R2和R3）中的GnRH-R转录物显著升高（P <0.05），但生长激素细胞和促黄体激素细胞中的GnRH-R转录物不变。因此，FSH和MSH细胞是这两种生殖状态所必需的，而其他垂体细胞仅在睾丸成熟期间被招募。促性腺激素释放激素受体在促性腺激素细胞和非促性腺激素细胞中的差异表达表明了控制正常性发育的机制在细胞和功能上的异质性。
The role of multiple gonadotropin-releasing hormone receptor (GnRH-R) types in the regulation of gonadotropic and nongonadotropic cells remains speculative. To address this issue, we developed a technology integrating laser-captured microdissection of single digoxigenin-labeled pituitary cells coupled with real-time quantitative PCR to examine the expression profiles of three endogenous GnRH-R types (R1, R2, and R3) in immature and mature males of tilapia Oreochromis niloticus. Here, in addition to gonadotropes (luteinizing and folicle-stimulating hormone, FSH), we show GnRH-Rs are also present in lactotropes, somatotropes, thyrotropes, melanotropes (melanocyte-stimulating hormone, MSH), corticotropes and somatolactin cells. Subpopulations of pituitary cells express single (42.9%), multiple (32.4%) or lack (24.7%) GnRH-Rs. In immature males, the percentage of FSH cells containing combinations of GnRH-Rs was significantly higher (R1+R2: 24%, P <0.05; R1+R2+R3: 25%, P <0.01) than in mature males, whereas the percentage showing only R1 and R1 and R3 transcripts (P <0.05) was higher in mature males. Significantly greater copies of R1 and R3 transcripts were found in MSH cells of immature and mature males, respectively (P <0.05). GnRH-R transcripts in other pituitary cells (lactotropes, R1 and R2; somatolactin cells/thyrotropes/corticotropes, R1, R2, and R3) were significantly higher in mature males (P <0.05) but were unaltered in somatotropes and luteinizing hormone cells. Thus, FSH and MSH cells are required for both reproductive states, whereas other pituitary cells are recruited only during testicular maturation. The differential expression of GnRH-Rs in gonadotropic and nongonadotropic cells demonstrates cellular and functional heterogeneity of mechanisms controlling normal sexual development.