Anti-Cancer Effect of α-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells
Anti-Cancer Effect of α-Solanine by Down-Regulating S100P Expression in Colorectal Cancer Cells
复制标题
α-茄碱通过下调结直肠癌细胞中 S100P 表达的抗癌作用。
DOI：
10.2174/1574892813666180329163945
复制
发表时间：
2018-01-01
影响因子：
2.8
通讯作者：
Jiang, Lei
中科院分区：
文献类型：
--
作者：
Ni, Xiaofen;Chen, Jiajun;Jiang, Lei
背景资料：α-龙葵碱是龙葵中最重要的活性成分，对多种癌细胞具有抗癌活性。目的：研究α-龙葵碱对结直肠癌细胞的体内、外抑癌作用，方法：采用CCK-8法检测细胞活力，Cycletest Plus DNA试剂盒检测细胞周期，MTT法检测细胞增殖，流式细胞仪检测细胞周期，流式细胞仪检测细胞凋亡。流式细胞仪检测细胞凋亡; Transwell法检测细胞迁移和侵袭能力; Western blotting法检测S100 P蛋白表达;慢病毒载体表达靶向S100 P基因的shRNA。我们证明，α-龙葵碱抑制CRC细胞（SW 480，SW 620和HT-29）的生长以及迁移和侵袭，诱导细胞周期阻滞和凋亡在体外，并抑制肿瘤生长在体内。此外，我们观察到S100 P的表达被α-龙葵碱下调。过表达S100 P可部分逆转α-龙葵碱对大肠癌细胞的生长抑制作用。结论：α-龙葵碱可能是一种潜在的治疗大肠癌的药物，α-龙葵碱对大肠癌细胞的抗肿瘤作用可能至少部分是通过下调S100 P来介导的。
Background: alpha-Solanine, the most important and active component of Solanum nigrum, was found to have anti-cancer activity on multiple cancer cells. However, its effects on colorectal cancer (CRC) and associated molecular mechanisms remain to be further elucidated.Objective: In this study, we investigated the anti-cancer effects of alpha-solanine against CRC cells in vitro and in vivo.Materials & Methods: Cell viability was measured using Cell Counting Kit-8 (CCK-8) assay; cell cycle was analyzed with a Cycletest Plus DNA Reagent Kit; cell apoptosis was detected by flow cytometer; cell migration and invasive ability was determined by Transwell assays; S100P protein expression was also analyzed by western blotting; lentiviral vectors expressing shRNA targeting the S100P gene.Results: We demonstrated that alpha-solanine inhibited CRC cell (SW480, SW620 and HT-29) growth as well as migration and invasion, induced cell cycle arrest and apoptosis in vitro, and suppressed tumor growth in vivo. Moreover, we observed that S100P expression was downregulated by alpha-solanine. Overexpression of S100P partially reversed the a-solanine-induced growth inhibition of CRC cells. Conversely, knockdown of S100P by lentiviral-mediated RNAi resulted in significantly promoting the a-solanine-induced growth inhibition.Conclusion: These findings suggest that alpha-solanine is a potential agent for the treatment of CRC, and the anti-tumor effect of alpha-solanine in the CRC cells may be mediated at least partly by the downregulation of S100P.