The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo
The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo
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DOI：
10.1016/s0012-1606(02)00095-7
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发表时间：
2003-03-15
影响因子：
2.7
通讯作者：
West, JD
中科院分区：
文献类型：
--
作者：
Collinson, JM;Quinn, JC;West, JD
通过分析Pax6(+/+)Pax6(-/-)嵌合体中Pax6(-/-)细胞的基因表达和分布，探讨Pax6在小鼠眼和嗅觉上皮细胞中的作用。研究发现，在胚胎发育至E10.5天和E16.5天之间，Pax6自主地被要求细胞不仅向高水平表达Pax6的角膜上皮细胞，而且向角膜基质和内皮细胞充分贡献Pax6蛋白，在角膜基质和内皮细胞中检测到非常低的蛋白水平。Pax6(-/-)细胞对神经视网膜的贡献很小，在E16.5形成了小的细胞团，通常局限于神经节细胞层。视网膜色素上皮中的Pax6(-/-)细胞在E14.5时可表达着色途径的成分Trp2，在E16.5时少数细胞继续分化并产生色素。突变细胞与鼻上皮的分离和几乎排斥反映了突变细胞在其他发育环境中的行为，特别是晶状体，这表明共同的原发缺陷可能是导致不同Pax6相关表型的原因。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
The roles of Pax6 were investigated in the murine eye and the olfactory epithelium by analysing gene expression and distribution of Pax6(-/-) cells in Pax6(+/+) Pax6(-/-) chimeras. It was found that between embryonic days E10.5 and E16.5 Pax6 is autonomously required for cells to contribute fully not only to the corneal epithelium, where Pax6 is expressed at high levels, but also to the to the corneal stroma and endothelium, where the protein is detected at very low levels. Pax6(-/-) cells contributed only poorly to the neural retina, forming small clumps of cells that were normally restricted to the ganglion cell layer at E16.5. Pax6(-/-) cells in the retinal pigment epithelium could express Trp2, a component of the pigmentation pathway, at E14.5 and a small number went on to differentiate and produce pigment at E16.5. The segregation and near-exclusion of mutant cells from the nasal epithelium mirrored the behaviour of mutant cells in other developmental contexts, particularly the lens, suggesting that common primary defects may be responsible for diverse Pax6-related phenotypes. (C) 2003 Elsevier Science (USA). All rights reserved.