Survival of Escherichia coli in the intestine of fish
Survival of Escherichia coli in the intestine of fish
复制标题
DOI：
10.1046/j.1365-2109.1997.t01-1-00854.x
复制
发表时间：
1997-04-01
影响因子：
2
通讯作者：
Millar, SD
中科院分区：
文献类型：
--
作者：
Del Rio-Rodriguez, RE;Inglis, V;Millar, SD
研究了大肠杆菌在虹鳟（Oncorhynchus mykiss（Walbaum））肠道中的建立和持久性。感染是通过摄入受污染的饲料而不是通过浴暴露实现的。在15 ℃时，E.大肠杆菌在鱼的肠道中的数量在最初下降后增加，并且在4天后仍然可以检测到，在6 ℃下检测到2天，但是数量稳定下降。当提取的肠内容物接种E.体外培养大肠杆菌;在最初的下降之后，细菌生长恢复。离体肠内容物也是质粒介导抗性转移的适宜环境。土霉素抗性从杀鲑气单胞菌成功转移到大肠杆菌。在体内不能复制，可能与A.每条鱼获得的杀鲑剂不够高，因为它不能很好地经受饲料颗粒的干燥。还尝试了通过管饲法引入，但fa：导致建立了A.沙门氏菌感染这些结果表明，在15摄氏度左右的温度下，E.鱼类中的大肠杆菌不一定是最近通过受污染沃茨水域的指标，它可能是许多天前（也许是一段距离前）建立的感染的结果。此外，尽管鱼类病原体的抗生素耐药性确实发生了转移。在鱼肠道内容物的环境中，这是可能影响人类的细菌获得耐药性的高风险途径的可能性很低。
Establishment and persistence of Escherichia coli in the intestine of rainbow trout, Oncorhynchus mykiss (Walbaum), was investigated. Infection was achieved by ingestion of contaminated feed but not by bath exposure. At 15 degrees C, E. coli was found to increase in number in the intestine of fish after an initial decline, and could still be detected after 4 days, At 6 degrees C it was detected for 2 days but the numbers declined steadily. A similar trend was observed when extracted gut content was inoculated with E. coli in vitro; after an initial decline, bacterial growth recovered. Gut content in vitro also was a suitable environment for plasmid-mediated transfer of resistance. Oxytetracycline resistance was transferred successfully from Aeromonas salmonicida to E. coli, This could not be reproduced in vivo, perhaps because the inoculum of A. salmonicida achieved per fish was not high enough as it did not withstand drying well on the feed pellets. Introduction by gavage was also tried but fa:led to establish an A. salmonicida infection. These results show that at temperatures around 15 degrees C:, the presence of E. coli in fish need not be an indicator of recent passage through polluted waters, It may be a consequence of infection established many days before, and perhaps some distance away, Furthermore, although transfer of antibiotic resistance from a fish pathogen did take place in the environment of fish intestinal content, the likelihood of this being a high-risk route for acquisition of resistance by bacteria that can affect humans is low.