A microfluidic approach for screening submicroliter volumes against multiple reagents by using preformed arrays of nanoliter plugs in a three-phase liquid/liquid/gas flow
A microfluidic approach for screening submicroliter volumes against multiple reagents by using preformed arrays of nanoliter plugs in a three-phase liquid/liquid/gas flow
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DOI：
10.1002/anie.200462857
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发表时间：
2005-01-01
影响因子：
16.6
通讯作者：
Ismagilov, RF
中科院分区：
文献类型：
--
作者：
Zheng, B;Ismagilov, RF
可以在三相液/液/气流动中预先形成不同成分的纳米级塞阵。这些阵列可以被输送到微流体通道中，以测试目标（见示意图），如蛋白质结晶和酶分析所示。
Plugging a gap in screening Arrays of nanoliter-sized plugs of different compositions can be preformed in a three-phase liquid/liquid/gas flow. The arrays can be transported into a microfluidic channel to test against a target (see schematic representation), as demonstrated in protein crystallization and an enzymatic assay.