Molecular Characteristics of NDM-5-Producing Escherichia coli from a Cat and a Dog in South Korea
Molecular Characteristics of NDM-5-Producing Escherichia coli from a Cat and a Dog in South Korea
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DOI：
10.1089/mdr.2019.0382
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发表时间：
2020-02-11
影响因子：
2.6
通讯作者：
Jeong, Seok Hoon
中科院分区：
文献类型：
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作者：
Hong, Jun Sung;Song, Wonkeun;Jeong, Seok Hoon
在韩国的伴侣动物中很少有报道称产生碳青霉烯酶的肠杆菌科细菌（CPE）。在本研究中，我们旨在调查 2019 年韩国不同兽医医院住院的一只狗和一只猫的直肠拭子中分离出的两种产生新德里金属-β-内酰胺酶 (NDM-5) 的大肠杆菌的分子特征和相关性。通过 Etest 和肉汤微量稀释方法测试抗菌敏感性。进行PCR和测序来检测抗菌药物耐药基因。进行质粒复制子分型和Southern印迹杂交，分别确定质粒的复制起点和bla(NDM-5)基因的位置。通过多位点序列分型 (MLST) 和脉冲场凝胶电泳 (PFGE) 评估了大肠杆菌分离株的宏观限制谱。两种碳青霉烯类抗性大肠杆菌分离株携带位于 IncX3 质粒上的 bla(NDM-5) 基因。 MLST 等位基因序列分析表明，这两个大肠杆菌分离株属于序列类型 410 (ST410)。经 PFGE 评估，从猫中分离出的产生 NDM-5 的大肠杆菌与之前报道的从 2017 年在同一家医院住院的一只狗中分离出的产生 NDM-5 的大肠杆菌 ST410 分离物具有高度克隆相似性 (94%)。 bla(NDM-5) 基因周围遗传环境的两种大肠杆菌分离物具有相同的结构： ISAba125-bla(NDM-5)-ble(MBL)-trpF-TAT-ISCR26。这项研究揭示了产生 NDM-5 的大肠杆菌 ST410 分离株在狗和猫之间的直接传播。这是韩国首次报告从猫身上分离出产生 NDM-5 碳青霉烯酶的大肠杆菌。
Carbapenemase-producing Enterobacteriaceae (CPE) have been very rarely reported in companion animals in South Korea. In this study, we aimed to investigate the molecular characteristics and relatedness of two New Delhi metallo-beta-lactamase (NDM-5)-producing Escherichia coli isolates from rectal swabs of a dog and a cat hospitalized in different veterinary hospitals in South Korea during 2019. Antimicrobial susceptibility was tested by the Etest and broth microdilution method. PCR and sequencing were performed to detect antimicrobial resistance genes. Plasmid replicon typing and Southern blotting hybridization were performed to determine the replication origin of the plasmid and location of the bla(NDM-5) gene, respectively. Their macrorestriction profiles for E. coli isolates were assessed by multilocus sequence typing (MLST) and pulse-field gel electrophoresis (PFGE). The two carbapenem-resistant E. coli isolates harbored the bla(NDM-5) gene located on the IncX3 plasmid. Allele sequence analysis for MLST showed that the two E. coli isolates were attributed to sequence type 410 (ST410). The NDM-5-producing E. coli isolate from the cat presented high clonal similarity (94%) assessed by PFGE to a previously reported NDM-5-producing E. coli ST410 isolate from a dog hospitalized in the same hospital in 2017. The two E. coli isolates for the genetic environment surrounding the bla(NDM-5) gene had the same structure: ISAba125-bla(NDM-5)-ble(MBL)-trpF-TAT-ISCR26. This study revealed a direct transmission of the NDM-5-producing E. coli ST410 isolate between a dog and a cat. This is the first report of NDM-5 carbapenemase-producing E. coli isolate from a cat in South Korea.