QUANTIFICATION OF METHYL FARNESOATE LEVELS IN HEMOLYMPH BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
QUANTIFICATION OF METHYL FARNESOATE LEVELS IN HEMOLYMPH BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0021-9673(01)88696-9
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发表时间：
1991-05-24
期刊：
影响因子：
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通讯作者：
TSUKIMURA, B
中科院分区：
文献类型：
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作者：
BORST, DW;TSUKIMURA, B
法呢酸甲酯（MF）是一种无环倍半萜，在甲壳类和昆虫的血淋巴和其他组织中被检测到。 本文介绍了一种快速、灵敏的测定甲壳动物血淋巴MF的方法。 血淋巴样品的提取物通过正相高效液相色谱法（5 μ m硅胶，250 × 4.6 mm I.D.，1.3%乙醚的己烷溶液），并通过UV（220 nm）检测。 该方法的检测限小于250 pg/ml。 该方法为研究甲壳类动物和其他节肢动物中MF的生理功能提供了参考。
Methyl farnesoate (MF) is an acyclic sesquiterpenoid that has been detected in hemolymph and other tissues of crustaceans and insects. This paper describes a rapid and sensitive method for measuring MF in crustacean hemolymph. Extracts of hemolymph samples were separated by normal-phase high-performance liquid chromatography (5-mu-m silica, 250 x 4.6 mm I.D., 1.3% diethyl ether in hexane) and detected by UV (220 nm). The limit of detection with this method was less than 250 pg/ml. This method should be useful for studying the physiological functions of MF in crustaceans and other arthropods.