Neutrophil-mediated oxidation of erythrocyte peroxiredoxin 2 as a potential marker of oxidative stress in inflammation
Neutrophil-mediated oxidation of erythrocyte peroxiredoxin 2 as a potential marker of oxidative stress in inflammation
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DOI：
10.1096/fj.13-227298
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发表时间：
2013-08-01
期刊：
影响因子：
4.8
通讯作者：
Winterbourn, Christine C.
中科院分区：
文献类型：
--
作者：
Bayer, Simone B.;Maghzal, Ghassan;Winterbourn, Christine C.
过氧化物氧还蛋白2（Peroxiredoxin 2，Prx 2）是红细胞中一种丰富的巯基蛋白。它在暴露于过氧化氢（H2O2）时容易氧化，并通过在其还原和二硫键形式之间循环提供抗氧化保护。为了测试Prx2氧化是否可能发生在嗜中性粒细胞被激活的病理情况下，我们将人红细胞暴露于刺激的嗜中性粒细胞，并通过非还原性凝胶的免疫印迹来测量Prx2氧化。对于佛波醇肉豆蔻酸酯乙酸酯，脂多糖或金黄色葡萄球菌Prx2二聚体从
Peroxiredoxin 2 (Prx2) is an abundant thiol protein in erythrocytes. It is oxidized readily on exposure to hydrogen peroxide (H2O2) and provides antioxidant protection by cycling between its reduced and disulfide-bonded forms. To test whether Prx2 oxidation could occur in pathological situations where neutrophils are activated, we exposed human erythrocytes to stimulated neutrophils and measured Prx2 oxidation by immunoblotting of nonreducing gels. With phorbol myristate acetate, lipopolysaccharide or Staphylococcus aureus Prx2 dimer increased from