Single-Cell Protein Secretion Detection and Profiling
Single-Cell Protein Secretion Detection and Profiling
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DOI：
10.1146/annurev-anchem-061318-115055
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Fan, Rong
中科院分区：
文献类型：
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作者：
Chen, Zhuo;Chen, Jonathan J.;Fan, Rong
分泌蛋白质在介导各种生物学过程中发挥重要作用，如细胞间通讯、分化、迁移和群体或组织水平的稳态。在这里，我们审查生物分析技术和设备检测蛋白质分泌从单细胞。我们开始讨论传统的方法，然后详细介绍了最新的进展，在微工程系统检测单细胞蛋白质分泌的多重测量的重点。这些平台包括液滴微流控、基于微/细胞的测定和基于微室的测定，其中比较了优点和局限性。微尺度系统还可以跟踪单细胞中的蛋白质分泌动力学，进一步增强了对细胞间通讯网络的研究。展望未来，我们讨论了剩余的挑战和未来的机遇，这些挑战和机遇将改变系统水平上细胞分泌功能的基础研究以及免疫监测和癌症治疗的临床应用。
Secreted proteins play important roles in mediating various biological processes such as cell-cell communication, differentiation, migration, and homeostasis at the population or tissue level. Here, we review bioanalytical technologies and devices for detecting protein secretions from single cells. We begin by discussing conventional approaches followed by detailing the latest advances in microengineered systems for detecting single-cell protein secretions with an emphasis on multiplex measurement. These platforms include droplet microfluidics, micro-/nanowell-based assays, and microchamber-based assays, among which the advantages and limitations are compared. Microscale systems also enable the tracking of protein secretion dynamics in single cells, further empowering the study of the cell-cell communication network. Looking forward, we discuss the remaining challenges and future opportunities that will transform basic research of cellular secretion functions at the systems level and the clinical applications for immune monitoring and cancer treatment.