Methylation of the alphafetoprotein gene in cell populations isolated from rat livers during carcinogenesis.
Methylation of the alphafetoprotein gene in cell populations isolated from rat livers during carcinogenesis.
复制标题
致癌过程中从大鼠肝脏分离的细胞群中甲胎蛋白基因的甲基化。
DOI：
10.1093/nar/13.22.8105
复制
发表时间：
1985
影响因子：
14.9
通讯作者：
Fausto,N
中科院分区：
文献类型：
--
作者：
Petropoulos,CJ;Yaswen,P;Panzica,M;Fausto,N
我们检查了甲胎蛋白基因的甲基化模式和组织在全肝和分离细胞群纯化的肝脏喂食致癌饮食干扰DNA甲基化。利用限制性内切酶酶切，我们发现在癌变早期分离的卵形细胞（AFP产生者）和肝细胞（非AFP产生者）中AFP基因的甲基化模式和整体组织没有差异。我们的研究表明，在产生AFP的细胞群中以及在AFP合成不活跃的细胞中，AFP基因的大多数CCGG位点都被广泛甲基化。此外，我们描述了Sprague-Dawley大鼠AFP和白蛋白基因多态性的存在。
We examined the methylation pattern and organization of the AFP gene in whole livers and in isolated cell populations purified from livers of rats fed a carcinogenic diet which interferes with DNA methylation. Using restriction endonuclease digestion, we find no differences in methylation pattern and overall organization of the AFP gene in oval cells (AFP-producers) and hepatocytes (non-producers) isolated at the early stages of carcinogenesis. Our studies indicate that in cell populations which produce AFP as well as in cells which are not active in AFP synthesis, the majority of the CCGG sites of the AFP gene are extensively methylated. In addition, we describe the existence of polymorphism in the AFP and albumin genes of Sprague-Dawley rats.
重组 DNA 和细胞增殖
DOI：
10.1016/0378-1119(87)90443-4
发表时间：
1984
期刊：
Self-Assembly
影响因子：
--
作者：
G. Stein;J. Stein
通讯作者：
J. Stein