New Amniotic Membrane Based Biocomposite for Future Application in Reconstructive Urology.
New Amniotic Membrane Based Biocomposite for Future Application in Reconstructive Urology.
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DOI：
10.1371/journal.pone.0146012
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Drewa T
中科院分区：
文献类型：
--
作者：
Adamowicz J;Pokrywczyńska M;Tworkiewicz J;Kowalczyk T;van Breda SV;Tyloch D;Kloskowski T;Bodnar M;Skopinska-Wisniewska J;Marszałek A;Frontczak-Baniewicz M;Kowalewski TA;Drewa T
由于羊膜（Am）支持上皮再生和抑制瘢痕形成的能力，Am有可能成为重建泌尿学的重要资产，即，输尿管和尿道重建术。由于其力学性能较差，限制了其在泌尿外科重建中的应用。AM增强静电纺丝纳米纤维提供了一种新的策略，以提高AM的机械阻力，而不影响其独特的生物活性。本研究评估了由Am和纳米纤维组成的生物复合材料作为大鼠模型膀胱增大的移植物。三明治结构的生物复合材料由冷冻的Am构成，并在两侧覆盖由电纺聚-（L-丙交酯-co-E-己内酯）（PLCL）制备的双层膜。Wistar大鼠进行半膀胱和膀胱扩张与生物复合材料。免疫组织化学分析（苏木精和伊红[H&E]，抗smoothelin和Masson三色染色[TRI]）显示尿路上皮和平滑肌层的有效再生。抗smoothelin染色证实了新膀胱壁内存在收缩性平滑肌。三明治结构的生物复合移植物材料被设计用于再生膀胱壁，满足正常膀胱张力、收缩、弹性和顺应性的要求。基于杨氏弹性模量进行的再生膀胱壁的力学评价反映了膀胱增大部分的组织学重塑的变化。生物复合材料的结构使得将完整的Am输送到再生区域成为可能。未改性的Am表面支持膀胱壁的再生，并且PLCL膜没有干扰再生过程。AM增强静电纺丝纳米纤维提供了一种新的策略，以提高AM的机械阻力，而不影响其独特的生物活性。
Due to the capacity of the amniotic membrane (Am) to support re-epithelisation and inhibit scar formation, Am has a potential to become a considerable asset for reconstructive urology i.e., reconstruction of ureters and urethrae. The application of Am in reconstructive urology is limited due to a poor mechanical characteristic. Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance, without affecting its unique bioactivity profile. This study evaluated biocomposite material composed of Am and nanofibers as a graft for urinary bladder augmentation in a rat model. Sandwich-structured biocomposite material was constructed from frozen Am and covered on both sides with two-layered membranes prepared from electrospun poly-(L-lactide-co-E-caprolactone) (PLCL). Wistar rats underwent hemicystectomy and bladder augmentation with the biocomposite material. Immunohistohemical analysis (hematoxylin and eosin [H&E], anti-smoothelin and Masson’s trichrome staining [TRI]) revealed effective regeneration of the urothelial and smooth muscle layers. Anti-smoothelin staining confirmed the presence of contractile smooth muscle within a new bladder wall. Sandwich-structured biocomposite graft material was designed to regenerate the urinary bladder wall, fulfilling the requirements for normal bladder tension, contraction, elasticity and compliance. Mechanical evaluation of regenerated bladder wall conducted based on Young’s elastic modulus reflected changes in the histological remodeling of the augmented part of the bladder. The structure of the biocomposite material made it possible to deliver an intact Am to the area for regeneration. An unmodified Am surface supported regeneration of the urinary bladder wall and the PLCL membranes did not disturb the regeneration process. Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance without affecting its unique bioactivity profile.