BK virus salivary shedding and viremia in renal transplant recipients
BK virus salivary shedding and viremia in renal transplant recipients
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DOI：
10.1590/1678-7757-2018-0435
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发表时间：
2019-01-01
影响因子：
2.7
通讯作者：
Braz-Silva, Paulo Henrique
中科院分区：
文献类型：
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作者：
Sarmento, Dmitry José de Santana;Palmieri, Michelle;Braz-Silva, Paulo Henrique
目的：本研究旨在验证肾移植受者唾液中多瘤病毒BK（BKPyV）的存在，并将其与血液病毒血症相关联。材料和方法：我们进行了一项横断面研究，样本涉及126例肾移植受者。从这些患者中收集了126份唾液样品和52份血液样品。使用实时PCR进行BKPyV的检测和定量。为了比较血液和唾液中BKPyV的存在，使用二项式比例检验。为了验证唾液脱落BKPyV和移植后时期（以月计）之间的关联，使用Mann-Whitney检验。斯皮尔曼相关性被用来关联唾液中的病毒载量与肾移植受者的血液。结果如下：研究组的平均年龄为51.11 ± 12.45岁，69名参与者（54.8%）为女性，平均移植后时间为4.80 ± 6.04个月。在唾液和血液的几个样品中定量BKPyV，中位数分别为1，108 cp/mL和1，255 cp/mL。只有16/52（30.8%）的参与者在血液中出现BKPyV，59/126（46.8%）的参与者在唾液中排出病毒（p=0.004）。在较短的移植后时期（3.86 +/- 5.25，p=0.100），在患者中发现BKPyV脱落。在唾液和血液中的病毒定量之间观察到弱相关性（斯皮尔曼相关系数=0.193）。结论：这项研究的结果表明，虽然唾液分泌更多的BKPyV比血液，唾液脱落和血液病毒血症之间没有可靠的相关性，显示两个独立的病毒复制区室。
Objectives: This study aimed to verify the presence of polyomavirus BK (BKPyV) in the saliva of kidney transplant recipients and to correlate it with blood viremia. Material and Methods: We have conducted a cross-sectional study with a sample involving 126 renal transplant recipients. 126 samples of saliva and 52 samples of blood were collected from these patients. Detection and quantification of BKPyV were performed using a real-time PCR. To compare the presence of BKPyV in blood and saliva, the binomial proportion test was used. To verify associations between salivary shedding BKPyV and post-transplant periods (in months), the Mann-Whitney test was used. Spearman's correlation was used to correlate the viral load in the saliva with blood of kidney transplant recipients. Results: The mean age of the study group was 51.11 +/- 12.45 years old, and 69 participants (54.8%) were female, with a mean post-transplantation time of 4.80 +/- 6.04 months. BKPyV was quantified in several samples of saliva and blood, with medians of 1,108 cp/mL and 1,255 cp/mL, respectively. Only 16/52 (30.8%) participants presented BKPyV in blood, and 59/126 (46.8%) excreted the virus in saliva (p=0.004). BKPyV shedding was found in patients at a shorter post-transplantation period (3.86 +/- 5.25, p=0.100). A weak correlation was observed between viral quantification in saliva and blood (Spearman's correlation coefficient=0.193). Conclusion: The results of this study suggested that, although saliva excretes more BKPyV than blood, there is no reliable correlation between salivary shedding and blood viremia, showing two independent compartments of viral replication.