ANTIGENIC S-TYPE LIPOPOLYSACCHARIDE OF BRUCELLA-ABORTUS 1119-3
ANTIGENIC S-TYPE LIPOPOLYSACCHARIDE OF BRUCELLA-ABORTUS 1119-3
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DOI：
10.1128/iai.46.2.384-388.1984
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
DUNCAN, JR
中科院分区：
文献类型：
--
作者：
CAROFF, M;BUNDLE, DR;DUNCAN, JR
从B分离的抗原性酚相可溶性脂多糖。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、受控水解、高碘酸盐氧化、甲基化和1H和13 C NMR研究表明，通过热酚-水提取的牛流产杆菌1119-3是S型脂多糖，其可以被裂解以产生脂质A和O链多糖，其被鉴定为1，2-连接的4，6-二脱氧-4-甲酰胺基-α-氨基-N-甲基-N-（2-甲基-2-氧代-N-甲基-N-氧代- D-吡喃甘露糖残基。牛抗B血清学反应性。牛流产杆菌1119-3与小肠结肠炎耶尔森氏菌血清型O：9和霍乱弧菌的脂多糖的关系现在已经与1，2-连接的N-酰化的4-氨基-4，6-双脱氧-α-葡糖苷酶的出现有关。其脂多糖的O-链多糖中的D-吡喃甘露糖基单元。
Antigenic phenol-phase soluble lipopolysaccharide isolated from B. abortus 1119-3 by hot phenol-water extraction was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, controlled hydrolysis, periodate oxidation, methylation and 1H and 13C NMR studies to be an S-type lipopolysaccharide which could be cleaved to yield a lipid A and an O-chain polysaccharide identified as an unbranched linear homopolymer of 1,2-linked 4,6-dideoxy-4-formamido-.alpha.-D-mannopyranosyl residues. The serological reactivity of bovine antiserum to B. abortus 1119-3 with the lipopolysaccharides of Yersinia enterocolitica serotype O:9 and Vibrio cholerae has now been related to the occurrence of 1,2-linked N-acylated 4-amino-4,6-dideoxy-.alpha.-D-mannopyranosyl units in the O-chain polysaccharides of their lipopolysaccharides.