A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus.
A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus.
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表达 Ag85A-HA2 融合蛋白的新型 DNA 疫苗可提供针对甲型流感病毒和金黄色葡萄球菌的保护
DOI：
10.1186/1743-422x-10-40
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发表时间：
2013-01-31
期刊：
影响因子：
4.8
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Dai J;Pei D;Wang B;Kuang Y;Ren L;Cao K;Zuo B;Shao J;Li S;Jiang Z;Li H;Li M
继发性肺炎由金黄色葡萄球菌（S。金黄色葡萄球菌）引起显著的发病率和死亡率。本研究的目的是设计一种新的DNA疫苗，其编码结核分枝杆菌分泌的抗原Ag 85 A与甲型流感病毒（IAV）HA 2蛋白融合，以提供对流感和继发感染的保护。金黄色。经RT-PCR、Western blotting和免疫荧光分析证实，该DNA疫苗载体能在哺乳动物细胞中高效表达所编码的抗原。用该疫苗肌肉注射免疫小鼠，然后用IAV和S.金黄色。免疫小鼠肺组织和脾细胞培养IFN-γ水平显著高于各自的对照组。尽管HI试验中的抗体滴度较低，但用新型疫苗载体免疫的小鼠血清在体外中和试验中有效。该疫苗可减少IAV攻毒小鼠的体重损失。Western blotting和RT-PCR结果显示，疫苗能显著增强S.金黄色。TLR 2高表达小鼠（pEGFP/Ag 85 A-HA 2或iPR）感染S.金黄色。肺组织IL-10浓度和S.金黄色葡萄球菌攻击后，免疫小鼠的金黄色葡萄球菌滴度显著降低，TLR 2表达增加。金黄色。结果表明，Ag 85 A能增强小鼠对IAV和S. TLR 2参与宿主对金黄色葡萄球菌的免疫应答。金黄色。
Secondary pneumonia due to Staphylococcus aureus (S. aureus) causes significant morbidity and mortality. The aim of the research was designed a novel DNA vaccine encoding the Mycobacterium tuberculosis secreted antigen Ag85A fused with the influenza A virus (IAV) HA2 protein to provide protection against both influenza and secondary infection with S. aureus. The DNA vaccine vector efficiently expressed the encoded antigen in mammalian cells, as determined by RT-PCR, Western blotting and immunofluorescence analysis. Mice were immunized with the vaccine by intramuscular injection before challenge with IAV and S. aureus. The pulmonary and the splenocyte culture IFN-γ levels were significant higher in immunized mice than their respective controls. Although the antibody titer in the HI test was low, the sera of mice immunized with the novel vaccine vector were effective in neutralisation assay in vitro. The vaccine could reduce the loss of body weight in mice during IAV challenge. Both Western blotting and RT-PCR showed that the vaccine markedly enhanced toll like receptor 2 (TLR2) expression in splenocytes after the secondary infection with S. aureus. The survival rate of mice with high TLR2 expression (pEGFP/Ag85A-HA2 or iPR) was significantly increased compared with mice immunized with pEGFP/HA2 after challenge with S. aureus. However, the pulmonary IL-10 concentration and S. aureus titer were significantly decreased in immunized mice, and expression of TLR2 was increased after challenge with S. aureus. These results demonstrated that Ag85A could strengthen the immune response to IAV and S. aureus, and TLR2 was involved in the host response to S. aureus.