Ethanolic extract from bulbs of Cipura paludosa reduced long-lasting learning and memory deficits induced by prenatal methylmercury exposure in rats
Ethanolic extract from bulbs of Cipura paludosa reduced long-lasting learning and memory deficits induced by prenatal methylmercury exposure in rats
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DOI：
10.1016/j.dcn.2012.08.003
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发表时间：
2013-01-01
影响因子：
4.7
通讯作者：
Ferreira, Vania M.
中科院分区：
文献类型：
--
作者：
Lucena, Greice M.;Prediger, Rui D.;Ferreira, Vania M.
本课题组以前的研究表明，从巴西北部广泛分布的本土植物Cipua paludosa(Iridaceae)的鳞茎中提取的乙醇提取物(EE)和分离出的化合物具有重要的生物学特性。在本研究中，评估了长期使用EE对发育早期暴露于甲基汞(MeHg)的成年大鼠记忆的影响。孕鼠在妊娠第15天给予单剂量甲基汞(8 mg/kg)灌胃，这是皮质神经元增殖的关键发育期。连续给成年仔鼠灌胃沼泽沙鼠EE(1、10、100 mg/kg)，连续14天。EE以一种特定的方式改善了短期社会记忆，并促进了对短期和长期记忆的跳跃抑制性回避。甲基汞暴露导致显著的长期社会识别记忆损害，而EE改善了这种损害。此外，EE显著增加了胎儿期甲基汞暴露大鼠的跳台潜伏期，特别是在短期时段。这些结果表明，EE可减轻甲基汞暴露所致的长期短期学习记忆障碍。这些发现可能会鼓励进一步的研究，评估沼泽沙门氏菌及其成分在与环境污染物暴露相关的神经病理条件下的认知增强特性。(C)2012爱思唯尔有限公司。保留所有权利。
Previous studies from our group have indicated important biological properties of the ethanolic extract (EE) and isolated compounds from the bulbs of Cipura paludosa (Iridaceae), a native plant widely distributed in northern Brazil. In the present study, the effects of chronic treatment with the EE on the memory of adult rats exposed to methylmercury (MeHg) during early development were assessed. Pregnant rats were treated by gavage with a single dose of MeHg (8 mg/kg) on gestational day 15, the developmental stage critical for cortical neuron proliferation. Adult offspring were administered orally with the EE of C. paludosa (1, 10 or 100 mg/kg) over 14 consecutive days. EE improved short-term social memory in a specific manner and facilitated the step-down inhibitory avoidance of short- and long-term memory. MeHg exposure induced pronounced long-lasting impairments in social recognition memory that were improved by EE. Moreover, EE significantly increased the step-down latencies specifically during the short-term session in prenatal MeHg-exposed rats. These results demonstrate that EE reduced the long-lasting short-term learning and memory deficits induced by MeHg exposure. These findings may encourage further studies evaluating the cognitive enhancing properties of C. paludosa and its components on neuropathological conditions associated with exposure to environmental contaminants. (C) 2012 Elsevier Ltd. All rights reserved.