THE RELATIONSHIP OF DNA EXCISION REPAIR OF ULTRAVIOLET-INDUCED LESIONS TO THE MAXIMUM LIFE-SPAN OF MAMMALS
THE RELATIONSHIP OF DNA EXCISION REPAIR OF ULTRAVIOLET-INDUCED LESIONS TO THE MAXIMUM LIFE-SPAN OF MAMMALS
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DOI：
10.1016/0047-6374(81)90094-4
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发表时间：
1981-01-01
影响因子：
5.3
通讯作者：
REGAN, JD
中科院分区：
文献类型：
--
作者：
FRANCIS, AA;LEE, WH;REGAN, JD
环境中存在的物理和化学物质可能会损害哺乳动物的DNA。已知在某些情况下，这种损伤通过DNA切除修复过程来修复。这一过程已被广泛研究使用的紫外线照射损伤的修复作为一个模型系统。该系统和5-溴脱氧尿苷光解测定在这里被用来测量DNA切除修复细胞来自21种哺乳动物。细胞培养中观察到的DNA修复频率和修复区域的平均大小与所研究的哺乳动物的各种最长寿命有关。哺乳动物的寿命与紫外线照射后20-22 h测得的DNA切除修复位点数之间存在近似线性关系。观察到与线性关系的几个偏差，这些偏差在很大程度上仍然无法解释。修复区域的大小与测试哺乳动物的最大寿命无关。
Physical and chemical agents present in the environment can potentially damage mammalian DNA. Such damage is known in some cases to be repaired by the process of DNA excision repair. This process has been extensively studied using the repair of UV irradiation damage as a model system. This system and the 5-bromodeoxyuridine photolysis assay were used here to measure DNA excision repair in cells derived from 21 mammalian species. The DNA repair proficiencies and the average size of the repaired regions seen in the cell cultures were related with the various maximum life spans of the mammals studied. There was an approximate linear correlaton between life span of the mammals and the number of DNA excision repair sites measured 20-22 h following UV irradiation of the cell cultures. Several deviations from the linear relationship were observed which remain largely unexplained. The size of the repaired regions was not related to the maximum life spans of the mammals tested.