Two Closely Related Subclass II SnRK2 Protein Kinases Cooperatively Regulate Drought-Inducible Gene Expression
Two Closely Related Subclass II SnRK2 Protein Kinases Cooperatively Regulate Drought-Inducible Gene Expression
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DOI：
10.1093/pcp/pcq041
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发表时间：
2010-05-01
影响因子：
4.9
通讯作者：
Shinozaki, Kazuo
中科院分区：
文献类型：
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作者：
Mizoguchi, Masahide;Umezawa, Taishi;Shinozaki, Kazuo
SNF 1相关蛋白激酶2（SnRK 2）的亚类III组成员在拟南芥中的阿坝和渗透胁迫信号中发挥重要作用，然而，其他亚类的作用仍然难以捉摸。本研究构建了SRK 2C/SnR2.8和SRK 2F/SnRK2.7双突变体，以研究亚类II SnRK 2s的功能。基因芯片分析表明，SnRK 2s亚类调控的干旱响应基因涉及ABA响应元件结合转录因子（AREB/ABF）及其靶基因，定量逆转录PCR证实这些基因在srk 2cf中表达显著下调。本研究表明，亚类II SnRK 2在拟南芥干旱胁迫信号传导中也发挥重要作用。
The subclass III group of SNF1-related protein kinase 2 (SnRK2) members is known to play an important role in ABA and osmotic stress signaling in Arabidopsis; however, the roles of other subclasses remain elusive. Here, we established a double mutant of SRK2C/SnR2.8 and SRK2F/SnRK2.7 to investigate the functions of subclass II SnRK2s. Microarray analysis suggested that subclass II SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/ABF) and their targets, and quantitative reverse transcription PCR confirmed that those genes were down-regulated significantly in srk2cf. This study indicates that subclass II SnRK2s also play important roles in drought stress signaling in Arabidopsis.