Controlled release of vascular endothelial growth factor from spray-dried alginate microparticles in collagen-hydroxyapatite scaffolds for promoting vascularization and bone repair
Controlled release of vascular endothelial growth factor from spray-dried alginate microparticles in collagen-hydroxyapatite scaffolds for promoting vascularization and bone repair
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DOI：
10.1002/term.2013
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发表时间：
2017-04-01
影响因子：
3.3
通讯作者：
O'Brien, Fergal J.
中科院分区：
文献类型：
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作者：
Quinlan, Elaine;Lopez-Noriega, Adolfo;O'Brien, Fergal J.
当前组织工程方法的主要限制是创建可以成功与宿主整合的功能性血管化结构;由于缺血性坏死，这通常导致植入失败。为了克服这一点，本工作的目的是开发一种方法，将生长因子洗脱藻酸盐微粒（MP）到冷冻干燥，胶原蛋白为基础的支架。胶原-羟基磷灰石（CHA）支架，以前优化的骨再生，功能化的血管生成生长因子，血管内皮生长因子（VEGF）的持续交付，以促进血管生成和增强骨再生的目的。VEGF最初通过喷雾干燥包封在藻酸盐MP中，产生VEGF的颗粒。
A major limitation with current tissue-engineering approaches is creating functionally vascularized constructs that can successfully integrate with the host; this often leads to implant failure, due to avascular necrosis. In order to overcome this, the objective of the present work was to develop a method to incorporate growth factor-eluting alginate microparticles (MPs) into freeze-dried, collagen-based scaffolds. A collagen-hydroxyapatite (CHA) scaffold, previously optimized for bone regeneration, was functionalized for the sustained delivery of an angiogenic growth factor, vascular endothelial growth factor (VEGF), with the aim of facilitating angiogenesis and enhancing bone regeneration. VEGF was initially encapsulated in alginate MPs by spray-drying, producing particles of