Self recognition by T cells. I. Bystander killing of target cells bearing syngeneic MHC antigens.
Self recognition by T cells. I. Bystander killing of target cells bearing syngeneic MHC antigens.
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DOI：
10.1084/jem.170.1.59
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发表时间：
1989-07-01
期刊：
影响因子：
--
通讯作者：
Duke RC
中科院分区：
文献类型：
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作者：
Duke RC
激活的CTL可以杀死任何与其结合或与其结合的细胞。这一观察已被用于确定同种异体反应性CTL是否能够识别携带自身MHC的细胞。当被它们的特定目标激活时，来自4个不同特异性和来源的19个CTL克隆杀死了携带同基因但不含第三方MHC抗原的旁观者目标。使用来自MHC重组动物的靶细胞，同基因旁观者的杀戮被证明仅限于单个自身MHC编码的分子。这些结果首次明确证明，T细胞，或者更准确地说，CTL，能够在没有其特定抗原的情况下进行自我识别。我们的发现支持T细胞谱系选择是胸腺前体细胞成熟过程中阳性选择的结果，这些前体细胞的抗原受体与自身MHC的亲和力很低，但却是真实的。
Activated CTL can kill any cell to which they bind or by which they are bound. This observation has been used to determine whether alloreactive CTL can recognize cells bearing self-MHC. When activated by their specific targets, 19 CTL clones of 4 different specificities and origins killed bystander targets bearing syngeneic but not third-party MHC antigens. Using target cells derived from MHC-recombinant animals, syngeneic bystander killing was shown to be restricted to a single self MHC-encoded molecule. These results provide the first clear demonstration that T cells, or more precisely CTL, are capable of self recognition in the absence of their specific antigen. Our findings support the model that T cell repertoire selection occurs as a result of positive selection during maturation in the thymus of precursor cells whose antigen receptors have low but real affinity for self-MHC.