THE ACTION OF HUMAN C-3 IN SOLUBLE OR CROSS-LINKED FORM WITH RESTING AND ACTIVATED MURINE LYMPHOCYTES-B
THE ACTION OF HUMAN C-3 IN SOLUBLE OR CROSS-LINKED FORM WITH RESTING AND ACTIVATED MURINE LYMPHOCYTES-B
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DOI：
10.1002/eji.1830150214
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发表时间：
1985-01-01
影响因子：
5.4
通讯作者：
DIERICH, M
中科院分区：
文献类型：
--
作者：
ERDEI, A;MELCHERS, F;DIERICH, M
补体的第三组分C3参与刺激B淋巴细胞增殖和成熟以分泌IG。在血清替代培养物中，用活化或静息小鼠脾淋巴细胞重新研究这种活化的程度。人C3以可溶性或交联形式使用。在一定浓度范围内，可溶性C3以及琼脂糖结合的或戊二醛交联的C3对（C57 BL/6 Jx）的静息脾淋巴细胞是无活性的。DBA/2）F1、C3 H/HeJ和C57 BL/6 J nu/nu小鼠。脂多糖激活的脾细胞，丰富的B细胞母细胞，刺激固定和交联的C3，而他们不响应可溶性C3。再刺激的程度与脂多糖诱导的程度相当，并导致Ig分泌细胞的增殖和成熟增加。胚细胞的刺激似乎是C3特异性的，因为它可以被游离C3抑制。
The 3rd component of complement, C3, was implied in the stimulation of B lymphocytes to proliferation and maturation for Ig secretion. The extent of this activation was reinvestigated with either activated or resting murine splenic lymphocytes in serum-substituted cultures. Human C3 was used in either soluble or cross-linked form. Soluble as well as Sepharose-bound or glutaraldehyde-cross-linked C3, over a range of concentrations, was inactive with resting splenic lymphocytes of (C57BL/6J .times. DBA/2)F1, C3H/HeJ and C57BL/6J nu/nu mice. Lipopolysaccharide-activated spleen cells, enriched for B cell blasts, were stimulated by immobilized and cross-linked C3, while they did not respond to soluble C3. The extent of restimulation was comparable to that induced by lipopolysaccharide and resulted in both increased proliferation and maturation to Ig-secreting cells. The stimulation of the blast cells appears to be C3 specific, since it can be inhibited by free C3.